Purpose: The aim of the study is to report successful outcome (live births) after sperm sorting with annexin V-MACS on cryopreserved spermatozoa with high level of sperm DNA fragmentation from a cancer patient survivor.
Methods: Cryopreserved spermatozoa were sorted with annexin V-MACS prior to ICSI. Sperm DNA fragmentation was evaluated by SCSA(®) and TUNEL.
Results: The couple had two previous IVF/ICSI cycles failures using sperm cryopreserved before cancer treatment. On third ICSI cycle attempt results were as follow: pre-annexin V-MACS sperm quality: 10 × 10(6)/ml, 3.3 % progressive motility, 1 % normal forms, TUNEL: 72.5 % positive cells, SCSA(®): 76.6 % DFI. Post-annexin V-MACS sperm quality: 2.8 × 10(6)/ml, 10 % progressive motility, TUNEL: 58.8 % positive cells. Eight metaphase II oocytes were collected, 4 fertilized, 2 embryos were transferred on day 3 and healthy twins were born (1 boy, 1 girl).
Conclusions: Annexin V-MACS technique could be a potential tool to improve sperm quality on cryopreserved spermatozoa of cancer patient and improve ICSI outcome.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879941 | PMC |
| http://dx.doi.org/10.1007/s10815-013-0086-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Graphene oxide and silymarin combination: A novel approach to improving post-cryopreservation quality of ram sperm.
Cryobiology
January 2025
Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran; Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address:
Graphene oxide (GO) has been extensively studied for its diverse biomedical applications, including drug delivery, imaging, and tissue engineering. Silymarin, as a flavonoid complex derived from the milk thistle plant, has recently shown potential health benefits, particularly concerning reproductive health. This study aims to evaluate the effects of GO and silymarin supplementation, both individually and in combination, on the characteristics of frozen-thawed ram sperm.
View Article and Find Full Text PDFDecision-making in women who considered planned oocyte cryopreservation: decision satisfaction or regret?
Reprod Biomed Online
March 2024
Boston IVF - The Eugin Group, Waltham, MA, USA; Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Boston, MA, USA; Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Boston, MA, USA.
Research Question: Among women who considered planned oocyte cryopreservation, does decision regret differ between those who pursued planned oocyte cryopreservation and those who did not?
Design: A survey was e-mailed to all women who presented for an initial consultation for planned oocyte cryopreservation between January 2016 and December 2021 using a secure REDCap platform. The survey comprised questions on demographics, reproductive planning and the validated Decision Regret Scale (DRS). Univariable and multivariable models were fitted to compare decision regret in the group who had proceeded with planned oocyte cryopreservation with the group who had not.
NPC2, a seminal plasma protein with membrane cholesterol-binding ability, influences the cryotolerance and functionality of spermatozoa from Chino Santandereano bulls.
Anim Reprod Sci
December 2024
Faculty of Veterinary Medicine, University Antonio Nariño, Popayán, Colombia. Electronic address:
Despite Latin America's rich biodiversity, active genetic material conservation programs are scarce. This study investigates potential freezability markers in both sperm and seminal plasma (SP) in Chino Santandereano, a Colombian Creole breed. Thirty ejaculates from ten Chino Santandereano bulls were cryopreserved and subsequently classified as of good (GFE) or poor (PFE) freezability according to their post-thaw total sperm motility (TMOT) and plasma membrane integrity (PMI).
View Article and Find Full Text PDFInfluence of antifreeze protein III on canine sperm cryopreservation.
Theriogenology
January 2025
Veterinary Clinic for Reproductive Medicine and Neonatology, Justus Liebig-University of Giessen, Germany.
Sperm cryopreservation is crucial in reproductive biotechnology; however, the longevity of frozen and thawed semen is limited by the deterioration of sperm cell integrity. This study aimed to examine the effects of adding antifreeze protein III (AFP III) to the diluent, using samples from eight healthy mature dogs. The ejaculates were divided into aliquots and diluted with a standard Tris-fructose-egg yolk extender containing AFP III at concentrations of 0, 0.
View Article and Find Full Text PDFPRDX5 and PRDX6 translocation and oligomerization in bull sperm: a response to cryopreservation-induced oxidative stress.
Cell Commun Signal
January 2025
Institute of Animal Reproduction and Food Research, Olsztyn, Poland.
Cryopreservation of bull sperm, crucial for breeding and assisted reproduction, often reduces sperm quality due to oxidative stress. This study examines how oxidative stress during cryopreservation affects peroxiredoxin 5 (PRDX5) and peroxiredoxin 6 (PRDX6) proteins, leading to their translocation and oligomerization in bull sperm. Increased reactive oxygen species (ROS) and nitric oxide (NO) levels were linked to reduced mitochondrial potential, higher DNA fragmentation, and increased membrane fluidity, prompting PRDX5 to move intracellularly and PRDX6 to the cell membrane.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!