Hantaviruses are members of the family Bunyaviridae and are an emerging cause of disease worldwide with high lethality in the Americas. In Brazil, the diagnosis for hantaviruses is based on immunologic techniques associated with conventional RT-PCR. A novel one-step SYBR Green real-time RT-PCR was developed for the detection and quantitation of Araraquara (ARAV) and Rio Mamore hantavirus (RIOMV). The detection limit of assay was 10 copies/μL of RNA in vitro transcribed of segment S. The specificity of assay was evaluated by melting curve analysis, which showed that the Araraquara virus amplified product generated a melt peak at 80.83 ± 0.89 °C without generating primer-dimers or non-specific products. The assay was more sensitive than conventional RT-PCR and we detected two samples undetected by conventional RT-PCR. The one-step SYBR Green real-time quantitative RT-PCR is specific, sensible and reproducible, which makes it a powerful tool in both diagnostic applications and general research of ARAV and RIOMV and possibly other Brazilian hantaviruses.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798901 | PMC |
| http://dx.doi.org/10.3390/v5092272 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular detection and epidemiological distribution of poultry respiratory viral pathogens in commercial chicken flocks in Bangladesh.
Poult Sci
December 2024
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. Electronic address:
Respiratory viral infections have a considerable detrimental impact on animal health as well as significant financial consequences in the poultry industry. The primary aim of this study is to investigate the major pathogens involved in respiratory diseases of poultry, the co-infection rate, and their epidemiological distribution in commercial chicken farms in Bangladesh. From June 2022 to December 2023, 300 pooled samples (swabs from live birds, and respiratory tissues from dead birds) were collected from the selected poultry farms where respiratory outbreaks were noticed.
View Article and Find Full Text PDFExpression of TSPAN1 and its link to thyroid nodules: one step forward on the path to thyroid tumorigenesis biomarkers.
BMC Cancer
November 2024
Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, PO Box: 19395-4763, Tehran, Iran.
Background: Thyroid cancer is ranked as the most common malignancy within the endocrine system and the seventh most prevalent cancer in women globally. Thyroid malignancies require evaluating biomarkers capable of distinguishing between them for accurate diagnosis. We examined both mRNA and protein levels of TSPAN1 in plasma and tissue samples from individuals with thyroid nodules to aid this endeavour.
View Article and Find Full Text PDFDetection and Quantification of Soil-Borne Wheat Mosaic Virus, Soil-Borne Cereal Mosaic Virus and Japanese Soil-Borne Wheat Mosaic Virus by ELISA and One-Step SYBR Green Real-Time Quantitative RT-PCR.
Viruses
October 2024
Julius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11-12, 38104 Brunswick, Germany.
Article Synopsis
- Furoviruses, which include SBWMV, SBCMV, JSBWMV, and FBMV, cause significant crop damage and stunting in cereals due to their bipartite structure.
- Researchers developed SYBR green-based real-time quantitative RT-PCR assays to accurately detect and differentiate RNA1 and RNA2 of these viruses, although there's a risk of false positives with certain primers.
- The study also suggests using ELISA for initial screening and emphasizes the potential of their qRT-PCR method to advance research in virus resistance and host interactions.
A one-step low-cost molecular test for SARS-CoV-2 detection suitable for community testing using minimally processed saliva.
Biol Methods Protoc
May 2024
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, 2780-157, Portugal.
The gold standard for coronavirus disease 2019 diagnostic testing relies on RNA extraction from naso/oropharyngeal swab followed by amplification through reverse transcription-polymerase chain reaction (RT-PCR) with fluorogenic probes. While the test is extremely sensitive and specific, its high cost and the potential discomfort associated with specimen collection made it suboptimal for public health screening purposes. In this study, we developed an equally reliable, but cheaper and less invasive alternative test based on a one-step RT-PCR with the DNA-intercalating dye SYBR Green, which enables the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly from saliva samples or RNA isolated from nasopharyngeal (NP) swabs.
View Article and Find Full Text PDFBacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration.
Open Vet J
January 2024
Laboratory of Biotechnology, Department of Microbiology, Agriculture Research Centre (ARC), Animal Health Research Institute (AHRI), Zagazig, Egypt.
Background: Minced meat is a valuable source of nutrients, but it is vulnerable to contamination by microorganisms commonly present in the environment. In addition, there is a risk of adulteration with cheaper meat sources, which can be harmful to consumers.
Aim: It is crucial to identify meat adulteration with distinct microbiological analysis for legal, economic, religious, and public health purposes.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!