Electrochemical impedance spectroscopy as a method for electrical characterization of the bilayers formed from lipid-amino acid systems.

Bilayer lipid membranes composed of phosphatidylcholine and isoleucine or phosphatidylcholine and tyrosine were investigated using electrochemical impedance spectroscopy. Interaction between membrane components causes significant deviations from the additivity rule which can be explained by formation of the domain structures. The surface area of domains was calculated based on derived equations. We suggest that the stoichiometry of phosphatidylcholine-isoleucine domain is equal 3:1. In the case of tyrosine-modified phosphatidylcholine membranes, domain with stoichiometry 7:1 should be taken into consideration.