AI Article Synopsis
- p97, also known as VCP or CDC-48, is an AAA+ chaperone that forms a homo-hexameric ring and is essential for processes like the ubiquitin-proteasome pathway and autophagy.
- Mutations in the human version of p97 are linked to neurodegenerative diseases, emphasizing its importance in maintaining cellular function.
- Research on the N-D1 and D2 domains of CDC-48 using high-speed atomic force microscopy showed that ATP binding induces a rotational movement crucial for its function.
Article Abstract
p97 (also called VCP and CDC-48) is an AAA+ chaperone, which consists of a substrate/cofactor-binding N domain and two ATPase domains (D1 and D2), and forms a homo-hexameric ring. p97 plays crucial roles in a variety of cellular processes such as the ubiquitin-proteasome pathway, the endoplasmic reticulum-associated protein degradation, autophagy, and modulation of protein aggregates. Mutations in human p97 homolog VCP are linked to neurodegenerative diseases. The key mechanism of p97 in these various functions has been proposed to be the disassembly of protein complexes. To understand the molecular mechanism of p97, we studied the conformational changes of hexameric CDC-48.1, a Caenorhabditis elegans p97 homolog, using high-speed atomic force microscopy. In the presence of ATP, the N-D1 ring repeatedly rotates ~23 ± 8° clockwise and resets relative to the D2 ring. Mutational analysis reveals that this rotation is induced by ATP binding to the D2 domain.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.str.2013.08.017 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An integrated photonic engine for programmable atomic control.
Nat Commun
January 2025
Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.
Solutions for scalable, high-performance optical control are important for the development of scaled atom-based quantum technologies. Modulation of many individual optical beams is central to applying arbitrary gate and control sequences on arrays of atoms or atom-like systems. At telecom wavelengths, miniaturization of optical components via photonic integration has pushed the scale and performance of classical and quantum optics far beyond the limitations of bulk devices.
View Article and Find Full Text PDFPro-inflammatory S100A8 Protein Exhibits a Detergent-like Effect on Anionic Lipid Bilayers, as Imaged by High-Speed AFM.
ACS Appl Mater Interfaces
December 2024
Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio av. 7, Vilnius, LT-10257, Lithuania.
Neuronal cell death induced by cell membrane damage is one of the major hallmarks of neurodegenerative diseases. Neuroinflammation precedes the loss of neurons; however, whether and how inflammation-related proteins contribute to the loss of membrane integrity remains unknown. We employed a range of biophysical tools, including high-speed atomic force microscopy, fluorescence spectroscopy, and electrochemical impedance spectroscopy, to ascertain whether the pro-inflammatory protein S100A8 induces alterations in biomimetic lipid membranes upon interaction.
View Article and Find Full Text PDFSelf-Assembly of Human Fibrinogen into Microclot-Mimicking Antifibrinolytic Amyloid Fibrinogen Particles.
ACS Appl Bio Mater
December 2024
MOE Key Laboratory of Bio-Intelligent Manufacturing, Liaoning Key Laboratory of Molecular Recognition and Imaging, School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Recent clinical studies have highlighted the presence of microclots in the form of amyloid fibrinogen particles (AFPs) in plasma samples from Long COVID patients. However, the clinical significance of these abnormal, nonfibrillar self-assembly aggregates of human fibrinogen remains debated due to the limited understanding of their structural and biological characteristics. In this study, we present a method for generating mimetic microclots in vitro.
View Article and Find Full Text PDFGlobular-shaped Aβ oligomers have diverse mechanisms for promoting Aβ aggregations with the facilitation of fibril elongation.
Neurobiol Dis
December 2024
Department of Neurology, Kanazawa University Graduate School of Medical Sciences, 13-1, Kanazawa 920-8640, Japan. Electronic address:
The accumulation of amyloid β-proteins (Aβ) in the extracellular space, forming insoluble plaques, is a primary pathological process underlying Alzheimer's disease (AD). Among the various Aβ species that appear during Aβ aggregation, Aβ oligomers are considered the most neurotoxic form. However, the precise mechanisms of their molecular functions within the Aβ aggregation cascade have not been clarified so far.
View Article and Find Full Text PDFExperimental investigation on modes of spray formation, droplet size and size distribution in a spinning disc atomizer.
Front Plant Sci
December 2024
Key Laboratory of Plant Protection Equipment, Ministry of Agriculture and Rural Affairs, Jiangsu University, Zhenjiang, China.
The spinning disc atomizer is extensively utilized in agricultural spraying, with optimized operating conditions significantly enhancing atomization performance. In this paper, the atomization characteristics of a spinning disc were studied using photographs taken by a high-speed camera. Ethanol-water solutions were used at various flow rates and the disc speed was varied in a wide range.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!