AI Article Synopsis
Article Abstract
In this work, we present a novel 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) radical phosphoramidite building block, which can be attached to the 5'-terminus of nucleic acids. To investigate the paramagnetic relaxation enhancement (PRE) emanating from this radical center, we incorporated the TEMPO label into various types of RNAs. We measured proton PREs for selectively (13)C-isotope labeled nucleotides to derive long-range distance restraints in a short 15 nucleotide stem-loop model system, underscoring the potential of the 5'-TEMPO tag to determine long-range distance restraints for solution structure determination. We subsequently applied the distance-dependent relaxation enhancement induced by the nitroxide radical to discern two folding states in a bistable RNA. Finally, we investigated the fast conformational sampling of the HIV-1 TAR RNA, a paradigm for structural flexibility in nucleic acids. With PRE NMR in combination with molecular dynamics simulations, the structural plasticity of this RNA was analyzed in the absence and presence of the ligand L-argininamide.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870906 | PMC |
| http://dx.doi.org/10.1021/cb400589q | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluating the effect of a virtual reality digital therapeutic on maternal stress among expectant and postpartum Black and Latina mothers: a protocol paper.
Front Psychiatry
December 2024
Department of Informatics and Health Data Science, University of Miami Miller School of Medicine, Miami, FL, United States.
Background: Maternal mental health is vital to overall well-being, particularly among expectant and postpartum Black and Latina women who experience higher stress levels and mental health challenges. Traditional healthcare systems often fail to meet these needs, underscoring the need for innovative and accessible interventions. NurtureVR, a digital maternal mental health and educational program, leverages virtual reality to offer mindfulness, relaxation, and guided imagery to support these women.
View Article and Find Full Text PDFHydrogen sulfide-mediated inhibition of ROCK exerts a vasoprotective effecton ischemic brain injury.
Am J Physiol Cell Physiol
December 2024
Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.
As a gas molecule, hydrogen sulfide (HS) exerts neuroprotective effects. Despite its recognized importance, there remains a need for a deeper understanding of HS's impact on vascular smooth muscle cells and its role in ischemic brain injury. This study employs encompassing cultured primary cerebral vascular smooth muscle cells, oxygen-glucose deprivation/reoxygenation model, in vitro vascular tone assessments, in vivo middle cerebral artery occlusion and reperfusion experimentation in male rats, and the utilization of ROCK knockout, to unravel the intricate relationship between H2S and cerebrovascular diastolic function.
View Article and Find Full Text PDFRecent years have witnessed an intense effort to unravel magnetic field effects in electrocatalysis, as they can enhance the performance of common electrocatalysts. Both experimental and theoretical studies have shown that magnetic fields may affect, among others, the macroscopic spin-orbital ordering, charge transport, bubble release, and electron transfer kinetics. This paper highlights Electrochemical Impedance Spectroscopy (EIS) as a tool to analyze and separate the effects of magnetic field on both the oxygen reduction and evolution reactions at cobalt iron oxide electrodes.
View Article and Find Full Text PDFPredicting Magnetic Barriers in Lanthanide Complexes with Electrostatic Potential Charges.
J Phys Chem A
December 2024
Department of Chemical and Materials Engineering, University of Nevada Reno, Reno, Nevada 89557, United States.
Single-molecule magnets (SMMs) with slow relaxation of magnetization and blocking temperatures above that of liquid nitrogen are essential for practical applications in high-density data storage devices and quantum computers. A rapid and accurate prediction of the effective magnetic relaxation barrier () is needed to accelerate the discovery of high-performance SMMs. Using density functional theory and multireference calculations, we explored correlations between , partial atomic charges, and the anisotropic barrier for a series of sandwich-type lanthanide complexes containing cyclooctatetraene, substituted cyclopentadiene, phospholyl, boratabenzene, or borane ligands.
View Article and Find Full Text PDFOptimized MR pulse sequence for high-resolution brain 3D-T1ρ mapping with weighted spin-lock acquisitions.
Magn Reson Med
December 2024
Center for Biomedical Imaging, Department of Radiology, New York University Grossman School of Medicine, New York, New York, USA.
Purpose: To implement and evaluate the feasibility of brain spin-lattice relaxation in the rotating frame (T1ρ) mapping using a novel optimized pulse sequence that incorporates weighted spin-lock acquisitions, enabling high-resolution three-dimensional (3D) mapping.
Methods: The optimized variable flip-angle framework, previously proposed for knee T1ρ mapping, was enhanced by integrating weighted spin-lock acquisitions. This strategic combination significantly boosts signal-to-noise ratio (SNR) while reducing data acquisition time, facilitating high-resolution 3D-T1ρ mapping of the brain.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!