Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838137 | PMC |
| http://dx.doi.org/10.1128/JVI.02261-13 | DOI Listing |
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