[Reverse genetics platform construction of influenza pandemic virus strain].

Objective: Reverse genetics was used to construct the platform of flu pandemic strain A/California/07/2009 (H1N1).

Methods: Eight genes fragements were amplified and ligated with bidirectional vector, recombinant plasmids were co transfected to the 293 T cells and rescued the virus. Gene sequencing, antigenic analysis and growth property were used to evaluate the rescued virus.

Results: Rescued virus show the genes sequence correct, keep the same antigenicity and similar growth property compared with wild type virus.

Conclusion: The pandemic virus reverse genetics platform of A/California/07/2009 (H1N1) were built. Based on this platform, rescued virus hold the similarity of antigenicity and growth ability with wild type virus.