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Mapping cellular hierarchy by single-cell analysis of the cell surface repertoire. | LitMetric

Mapping cellular hierarchy by single-cell analysis of the cell surface repertoire.

Cell Stem Cell

Division of Pediatric Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA.

Published: October 2013

AI Article Synopsis

Article Abstract

Stem cell differentiation pathways are most often studied at the population level, whereas critical decisions are executed at the level of single cells. We have established a highly multiplexed, quantitative PCR assay to profile in an unbiased manner a panel of all commonly used cell surface markers (280 genes) from individual cells. With this method, we analyzed over 1,500 single cells throughout the mouse hematopoietic system and illustrate its utility for revealing important biological insights. The comprehensive single cell data set permits mapping of the mouse hematopoietic stem cell differentiation hierarchy by computational lineage progression analysis. Further profiling of 180 intracellular regulators enabled construction of a genetic network to assign the earliest differentiation event during hematopoietic lineage specification. Analysis of acute myeloid leukemia elicited by MLL-AF9 uncovered a distinct cellular hierarchy containing two independent self-renewing lineages with different clonal activities. The strategy has broad applicability in other cellular systems.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3845089PMC
http://dx.doi.org/10.1016/j.stem.2013.07.017DOI Listing

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