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Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping. | LitMetric
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Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping.

Marcelo Miyata Adolfo Carlos Barreto Santos Natália Helena Mendes Eunice Atsuko Cunha Fernando Augusto Fiúza de Melo Clarice Queico Fujimura Leite

Braz J Microbiol

Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas , Araraquara, SP , Brasil.

Published: April 2011

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769851PMC
http://dx.doi.org/10.1590/S1517-838220110002000045DOI Listing

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