Tannin acyl hydrolase commonly known as tannase is an industrially important enzyme having a wide range of applications, so there is always a scope for novel tannase with better characteristics. A newly isolated tannase-yielding fungal strain identified as Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues like amla (Phyllanthus emblica), ber (Zyzyphus mauritiana), jamun (Syzygium cumini), Jamoa (Eugenia cuspidate) and keekar (Acacia nilotica) leaves. Among these substrates, maximal extracellular tannase production i.e. 170.75 U/gds and 165.56 U/gds was obtained with jamun and keekar leaves respectively at 28ºC after 96 h. A substrate to distilled water ratio of 1:2 (w/v) was found to be the best for tannase production. Supplementation of sodium nitrate (NaNO3) as nitrogen source had enhanced tannase production both in jamun and keekar leaves. Applications of the enzyme were studied in wine clarification and tea cream solubilization. It resulted in 38.05% reduction of tannic acid content in case of jamun wine, 43.59% reduction in case of grape wine and 74% reduction in the tea extract after 3 h at 35°C.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768918 | PMC |
| http://dx.doi.org/10.1590/S1517-83822011000100047 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cloning, purification and characterization of a novel thermostable recombinant tannase from Galactobacillus timonensis.
Enzyme Microb Technol
December 2024
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
The exorbitant production costs associated with natural tannases pose a significant challenge to their widespread industrial utilization. Microbial expression systems provide a cost-effective method for enzyme production. In this study, a putative gene encoding the subtype B tannase (Gt-Tan) was cloned from Galactobacillus timonensis and expressed heterologously in Escherichia coli BL21 (DE3) cells.
View Article and Find Full Text PDFEffects of cassava pellets and enzymes addition on growth performance, meat quality, serum biochemical indices and cecum microbiota of broilers.
Poult Sci
November 2024
Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. Electronic address:
Article Synopsis
- The study examined how different diets, particularly those including cassava pellets and enzyme supplements, affect the growth and gut microbiota of broiler chicks.
- Results showed that a diet with 10% cassava pellets significantly improved weight gain and meat quality compared to a basic diet.
- The addition of cassava pellets also enhanced the microbial composition in the gut, indicating potential benefits for poultry health and productivity.
Biotechnological potential of salt tolerant and xerophilic species of Aspergillus.
Appl Microbiol Biotechnol
November 2024
Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Uppsalaan 8, 3584 CT, Utrecht, The Netherlands.
Xerophilic fungi occupy versatile environments owing to their rich arsenal helping them successfully adapt to water constraints as a result of low relative humidity, high-osmolarity, and high-salinity conditions. The general term xerophilic fungi relates to organisms that tolerate and/or require reduced water activity, while halophilic and osmophilic are applied to specialized groups that require high salt concentrations or increased osmotic pressure, respectively. Species belonging to the family Aspergillaceae, and especially those classified in Aspergillus subgenus Aspergillus (sections Restricti and Aspergillus) and Polypaecilum, are particularly enriched in the group of osmophilic and salt-tolerant filamentous fungi.
View Article and Find Full Text PDFInterstrain Variability in the Production of Bioactive Phenolic Metabolites from Flavan-3-ols.
J Agric Food Chem
October 2024
Institut sur la Nutrition et les Aliments Fonctionnels de l'Université Laval, Faculté des Sciences de l'agriculture et de l'alimentation, Université Laval, Quebec G1 V 0A6, QC, Canada.
Flavan-3-ols intake is associated with numerous health benefits, but these are influenced by their conversion into smaller phenolic metabolites by the gut microbiota. Thus, the identification of bacteria that metabolize flavan-3-ols could lead to targeted interventions to enhance their benefits. To this end, we screened 47 strains for their ability to metabolize (+)-catechin, a flavan-3-ol.
View Article and Find Full Text PDFValorization of faba bean peels for fungal tannase production and its application in coffee tannin removal.
Food Chem X
October 2024
Food Science Department, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.
This study describes the optimization of the production conditions of tannase on unutilized food waste, green bean peels, using the central composite of the response surface methodology. It also focuses on applying purified tannase to reduce tannins in coffee. The proposed design recommended a temperature of 29.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!