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Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA). | LitMetric

Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA).

Braz J Microbiol

Laboratorio Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva, Faculdad Ciêcias Veterinarias , Universidad Nacional del Centro de la Pcia. de Buenos Aires , Tandil, Argentina.

Published: October 2009

AI Article Synopsis

Article Abstract

The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates studied, the panel of VNTR used made it possible to perform a first approach of the high genetic diversity of native strains of O157:H7 by MLVA.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768571PMC
http://dx.doi.org/10.1590/S1517-838220090004000026DOI Listing

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