The aim of this study was to investigate and compare the microbial community structures of eubacteria and archaea in the pit mud of Chinese Luzhou-flavor liquor from the wall (C(w)) and bottom (C(b)) of cellar through nested PCR-denaturing gradient gel electrophoresis (DGGE). The Shannon-Wiener index (H) calculated from the DGGE profiles showed that the community diversities of eubacteria and archaea in samples from C(b) were almost higher than that from C(w). In addition, cluster analysis of the DGGE profiles revealed that some differences were found in the microbial community structure in samples from different locations. The closely relative microorganisms of all eubacterial 16S rRNA gene sequences fell into four phyla (Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria), including 12 genera and 2 uncultured eubacteria. Moreover, 37.1% eubacteria were affiliated with Clostridium. Particularly, genus Acinetobacter was absent in all samples from C(b) but present in all samples from C(w). The closely relative microorganisms of all archaeal 16S rRNA gene sequences fell into four genera, which included Methanobrevibacter, Methanoculleus, Methanobacterium and Methanosaeta, while the dominant archaea in samples from C(w) and C(b) were similar. Results presented in this study provide further understanding of the spatial differences in microbial community structure in the pit mud, and is of great importance for the production and quality improvement of Luzhou-flavor liquor.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11274-013-1472-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Experimental study on optimization of pipe jacking mud mixture ratio based on MICP technology.
Sci Rep
December 2024
Nanyang Vocational College, Nanyang, 473000, China.
In the course of pipe jacking construction, the carrying-soil effect frequently arises, influenced by factors such as excavation unloading, ongoing disturbance from successive pipe sections, and the progressive accumulation of soil adhesion. The pipe jacking slurry serves as a critical agent for friction reduction and strata support, essential for the secure advancement of the construction process. This study introduces the Microbial-Induced Calcium Carbonate Precipitation (MICP) technology into the realm of pipe jacking slurry, aiming to enhance its friction-reduction capabilities and the stability of the soil enveloping the pipe.
View Article and Find Full Text PDFThe fight against malaria in Edo-North, Edo State, Nigeria: identifying risk factors for effective control.
PeerJ
December 2024
Department of Mathematical Statistics and Actuarial Sciences, University of the Free State, Bloemfontein, Free State, South Africa.
Background: This study investigated malaria epidemiology in Edo-North, Nigeria; a region within the equatorial rainforest belt that has lacked prior research on malaria prevalence. This research sought to investigate the prevalence of malaria and identify potential risk factors in Edo-North, Nigeria. Additionally, the study aimed to analyze trends in malaria cases to inform the development of effective malaria control measures.
View Article and Find Full Text PDFsp. nov. and sp. nov., two species isolated from fermentation cellar-producing sauce-flavour Chinese baijiu.
Int J Syst Evol Microbiol
November 2024
State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
is an important microbial component in pit mud due to its ability to produce alcohol and short-chain fatty acids. This study presents the characterization and taxonomy of two Gram-stain-positive, strictly anaerobic, rod-shaped mesophilic bacterial strains, designated MT-113 and MT-5, isolated from pit mud in a fermentation cellar used for producing sauce-flavour Chinese baijiu. Phylogenetic analysis based on genome and 16S rRNA gene sequences of strains MT-113 and MT-5 indicates their affiliation with the genus (Cluster I of the Clostridia), with FW431 and WLY-B-L2 as the closest related species.
View Article and Find Full Text PDFMetagenomics-based insights into the microbial community dynamics and flavor development potentiality of artificial and natural pit mud.
Food Microbiol
January 2025
State Key Laboratory of Food Science and Resources, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; Shaoxing Key Laboratory of Traditional Fermentation Food and Human Health, Jiangnan University (Shaoxing) Industrial Technology Research Institute, Shaoxing, 312000, China; National Engineering Research Center of Huangjiu, Zhejiang Guyuelongshan Shaoxing Wine CO., LTD, Shaoxing, 312000, Zhejiang, China. Electronic address:
Strong-flavor Baijiu (SFB) production has relied on pit mud (PM) as a starter culture. The maturation time of natural PM (NPM) is about 30 years, so artificial PM (APM) with a shorter maturation time has attracted widespread attention. This study reveals the microbial and functional dissimilarities of APM and NPM, and helps to elucidate the different metabolic roles of microbes during substrate degradation and flavor formation.
View Article and Find Full Text PDFCaproiciproducens converts lactic acid into caproic acid during Chinese strong-flavor Baijiu brewing.
Int J Food Microbiol
January 2025
National Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address:
Strong-flavor Baijiu, a type of Chinese liquor, is produced through anaerobic solid-state fermentation in a sealed mud pit. Ethyl caproate, the characteristic flavor compound of strong-flavor Baijiu, is influenced by caproic acid-producing bacteria in the pit mud. To better understand the formation of caproic acid, this study investigated the microbial composition and physicochemical parameters of pit mud from different layers (top, middle, and bottom) in Hubei and Sichuan provinces, China.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!