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Application of PNA openers for fluorescence-based detection of bacterial DNA. | LitMetric

Application of PNA openers for fluorescence-based detection of bacterial DNA.

Methods Mol Biol

Department of Biomedical Engineering, Boston University, Boston, MA, USA.

Published: March 2014

AI Article Synopsis

Article Abstract

Peptide nucleic acid (PNA) openers have the unique ability to invade double-stranded DNA with high efficiency and sequence specificity, making it possible to detect short (about 20 bp), single-copy bacterial DNA sequences. PNA openers bind to a target signature site on one strand of bacterial DNA, leaving the other strand open for hybridization with a circularizable oligonucleotide probe. The assembled complex serves as a template for rolling circle amplification. The obtained amplicon is decorated with short, single-stranded DNA probes carrying fluorophores and detected via fluorescence microscopy.

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Source
http://dx.doi.org/10.1007/978-1-62703-535-4_18DOI Listing

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