Oplopanax elatus is a medicinal plant on the verge of extinction because of overexploitation. In the present study, the effects of various factors on enhancing somatic embryogenesis and plantlet conversion were studied. Mature seeds were collected from a total of 13 plants from 4 mountains in South Korea, and the genetic distances were calculated to analyze the effect of genotype on somatic embryogenesis. Results of cluster analysis and the unweighted-pair-group method with arithmetic mean of 13 genotypes indicated the presence of 3 main groups. Both genotype and explant type affected the induction of somatic embryos (SEs). Sorak 2 and root were found to be the most suitable genotype and explant type, respectively, for SE induction in O. elatus. Among the different types of carbon sources tested, 5% sucrose induced the maximum number of SEs. The formation and development of SEs were significantly influenced by culture density; thus, 10 mg embryonic callus was found to be the most suitable for SE induction. The highest rates of germination and SE conversion were obtained in a germination medium containing 1.8 gelrite and 3.2 g·l(-1) agar. In addition, 80% of the plantlets that were transplanted into artificial soil acclimatized successfully. Thus, our results showed that the percentage survival of O. elatus during in vitro proliferation could be increased by optimizing to the somatic embryogenesis system.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766505 | PMC |
| http://dx.doi.org/10.1186/2193-1801-2-428 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regeneration, Regengrow and Tissue Repair in Animals: Evolution Indicates That No Regeneration Occurs in Terrestrial Environments but Only Recovery Healing.
J Dev Biol
December 2024
Comparative Histolab Padova, 35100 Padova, Italy.
The present, brief review paper summarizes previous studies on a new interpretation of the presence and absence of regeneration in invertebrates and vertebrates. Broad regeneration is considered exclusive of aquatic or amphibious animals with larval stages and metamorphosis, where also a patterning process is activated for whole-body regeneration or for epimorphosis. In contrast, terrestrial invertebrates and vertebrates can only repair injury or the loss of body parts through a variable "recovery healing" of tissues, regengrow or scarring.
View Article and Find Full Text PDFThe arabidopsis phytoglobin 1 (Pgb1) involvement in somatic embryogenesis is linked to changes in ethylene and the class VII ethylene transcription factor HRE2.
Planta
January 2025
Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Phytoglobin1 promotes Arabidopsis somatic embryogenesis through the mediation of ethylene and the ERFVII HRE2. Generation of somatic embryos in Arabidopsis (Arabidopsis thaliana) is a two-step process, encompassing an induction phase where embryogenic tissue (ET) is formed followed by a developmental phase encouraging the growth of the embryos. Using previously characterized transgenic lines dysregulating the class 1 Phytoglobin (Pgb1) we show that suppression of Pgb1 decreases somatic embryogenesis (SE).
View Article and Find Full Text PDFEfficient regeneration protocol for producing true-to-type oil palm ( (jacq.) through somatic embryogenesis from immature male inflorescence.
Heliyon
January 2025
ICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, India.
Producing homogeneous planting material in oil palm poses a significant challenge, which can be addressed through somatic embryogenesis. This study successfully achieved somatic embryogenesis using immature male inflorescence from Tenera hybrid. Modified Eeuwens medium yielded better results than the Murashige and Skoog (MS) and CHU (N6) media when supplemented with 2,4-D, picloram and α-naphthaleneacetic acid (NAA).
View Article and Find Full Text PDFTranscriptomic dynamics and cell-to-cell communication during the transition of prospermatogonia to spermatogonia revealed at single-cell resolution.
BMC Genomics
January 2025
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810008, China.
Background: Spermatogonia are essential for the continual production of sperm and regeneration of the entire spermatogenic lineage after injury. In mammals, spermatogonia are formed in the neonatal testis from prospermatogonia (also termed gonocytes), which are established from primordial germ cells during fetal development. Currently, the molecular regulation of the prospermatogonial to spermatogonia transition is not fully understood.
View Article and Find Full Text PDFTemporal and regional X-linked gene reactivation in the mouse germline reveals site-specific retention of epigenetic silencing.
Nat Struct Mol Biol
January 2025
IGMM, University of Montpellier, CNRS, Montpellier, France.
Random X-chromosome inactivation is a hallmark of female mammalian somatic cells. This epigenetic mechanism, mediated by the long noncoding RNA Xist, occurs in the early embryo and is stably maintained throughout life, although inactivation is lost during primordial germ cell (PGC) development. Using a combination of single-cell allele-specific RNA sequencing and low-input chromatin profiling on developing mouse PGCs, we provide a detailed map of X-linked gene reactivation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!