Trichothecene 3-O-acetyltransferase (TRI101) is an indispensable enzyme for the biosynthesis of trichothecenes, a group of mycotoxins produced by Fusarium graminearum. In this study, an inhibitor of TRI101 was identified by chemical array analysis using compounds from the RIKEN Natural Products Depository (NPDepo) library. Although the addition of the identified enzyme inhibitor to the fungal culture did not inhibit trichothecene production, it can serve as a candidate lead compound in the development of a mycotoxin inhibitor that inactivates fungal defense mechanisms.
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Reduction of Fusarium head blight and trichothecene contamination in transgenic wheat expressing trichothecene 3--acetyltransferase.
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Mycotoxin Prevention and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, Peoria, IL, United States.
, the causal agent of Fusarium head blight (FHB), produces various mycotoxins that contaminate wheat grains and cause profound health problems in humans and animals. Deoxynivalenol (DON) is the most common trichothecene found in contaminated grains. Our previous study showed that Arabidopsis-expressing trichothecene 3--acetyltransferase () converted DON to 3-acetyldeoxynivalenol (3-ADON) and excreted it outside of Arabidopsis cells.
View Article and Find Full Text PDFDetoxification and Excretion of Trichothecenes in Transgenic Expressing Trichothecene 3--acetyltransferase.
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April 2021
USDA, Agricultural Research Service, Mycotoxin Prevention and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, 1815 N. University street, Peoria, IL 61604, USA.
, the causal agent of Fusarium head blight (FHB), produces trichothecenes including deoxynivalenol (DON), nivalenol (NIV), and 3,7,15-trihydroxy-12,13-epoxytrichothec-9-ene (NX-3). These toxins contaminate grains and cause profound health problems in humans and animals. To explore exploiting a fungal self-protection mechanism in plants, we examined the ability of trichothecene 3--acetyltransferase () to detoxify several key trichothecenes produced by : DON, 15-ADON, NX-3, and NIV.
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Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G5C9, Canada.
Deoxynivalenol (DON) is a secondary fungal metabolite and contaminant mycotoxin that is widely detected in wheat and corn products cultivated around the world. Bio-remediation methods have been extensively studied in the past two decades and promising ways to reduce DON-associated toxicities have been reported. Bacterial epimerization of DON at the C3 carbon was recently reported to induce a significant loss in the bio-toxicity of the resulting stereoisomer (3-epi-DON) in comparison to the parental compound, DON.
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Institute of Plant Protection, Henan Academy of Agricultural Sciences; Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou 450002, Henan, China.
Wheat (Triticum aestivum L.), the most widely grown winter cereal crop in China, was grown on 24.3 million hectares in 2012.
View Article and Find Full Text PDFIdentification and characterization of an inhibitor of trichothecene 3-O-acetyltransferase, TRI101, by the chemical array approach.
Biosci Biotechnol Biochem
April 2014
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University.
Trichothecene 3-O-acetyltransferase (TRI101) is an indispensable enzyme for the biosynthesis of trichothecenes, a group of mycotoxins produced by Fusarium graminearum. In this study, an inhibitor of TRI101 was identified by chemical array analysis using compounds from the RIKEN Natural Products Depository (NPDepo) library. Although the addition of the identified enzyme inhibitor to the fungal culture did not inhibit trichothecene production, it can serve as a candidate lead compound in the development of a mycotoxin inhibitor that inactivates fungal defense mechanisms.
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