Improved sample preparation techniques and increasingly sensitive mass spectrometry (MS) analysis have revolutionized the study of protein post-translational modifications (PTMs) (Rush et al., Nat Biotechnol 23:94-101, 2005). Here, we describe a general approach for immunopurification and MS-based identification of acetylated proteins in biological samples. This approach is useful to characterize changes in the acetylome in response to biological interventions (Schwer et al., Aging Cell 8:604-606, 2009).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954751 | PMC |
| http://dx.doi.org/10.1007/978-1-62703-637-5_6 | DOI Listing |
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