Candida albicans is an opportunistic yeast that can cause oral candidosis through the formation of a biofilm, an important virulence factor that compromises the action of antifungal agents. The objective of this study was to compare the effect of rose bengal (RB)- and eosin Y (EY)-mediated photodynamic inactivation (PDI) using a green light-emitting diode (LED; 532 ± 10 nm) on planktonic cells and biofilms of C. albicans (ATCC 18804). Planktonic cultures were treated with photosensitizers at concentrations ranging from 0.78 to 400 μM, and biofilms were treated with 200 μM of photosensitizers. The number of colony-forming unit per milliliter (CFU/mL) was compared by analysis of variance and Tukey's test (P ≤ 0.05). After treatment, one biofilm specimen of the control and PDI groups were examined by scanning electron microscopy. The photosensitizers (6.25, 25, 50, 200, and 400 μM of EY, and 6.25 μM of RB or higher) significantly reduced the number of CFU/mL in the PDI groups when compared to the control group. With respect to biofilm formation, RB- and EY-mediated PDI promoted reductions of 0.22 log10 and 0.45 log10, respectively. Scanning electron microscopy showed that the two photosensitizers reduced fungal structures. In conclusion, EY- and RB-mediated PDI using LED irradiation significantly reduced C. albicans planktonic cells and biofilms.
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http://dx.doi.org/10.1007/s10103-013-1435-x | DOI Listing |
Sci Rep
December 2024
Department of Food Science and Technology, Sindos Campus, International Hellenic University, 57400, Thessaloniki, Greece.
Microalgae, have emerged as a potentially promising feed additive option due to their beneficial nutritional profile rich in bioactive compounds. The present study examines the incorporation of Chlorella sorokiniana (at 0.1% and 1%) into chicken feed compared to control feed and its effect on growth and health parameters of poultry grown at pilot plant scale.
View Article and Find Full Text PDFNanotechnol Sci Appl
December 2024
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland.
Purpose: Biofilms are one of the main threats related to bacteria. Owing to their complex structure, in which bacteria are embedded in the extracellular matrix, they are extremely challenging to eradicate, especially since they can inhabit both biotic and abiotic surfaces. This study aimed to create an effective antibiofilm nanofilm based on graphene oxide-metal nanoparticles (GOM-NPs).
View Article and Find Full Text PDFMicrobiol Res
December 2024
Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India. Electronic address:
Bacteria commonly live in a spatially organized biofilm assemblage. The metabolic activity inside the biofilm leads to segmented physiological microenvironments. In nature, bacteria possess several pleomorphic forms to withstand certain ecological alterations.
View Article and Find Full Text PDFMar Drugs
December 2024
Interdepartmental Centre of Environmental Science and Engineering (CINSA), University of Cagliari, Via San Giorgio 12, 09124 Cagliari, Italy.
The green synthesis of silver (Ag) and zinc oxide (ZnO) nanoparticles (NPs), as well as Ag/AgO/ZnO nanocomposites (NCs), using polar and apolar extracts of , offers a sustainable method for producing nanomaterials with tunable properties. The impact of the synthesis environment and the nanomaterials' characteristics on cytotoxicity was evaluated by examining reactive species production and their effects on mitochondrial bioenergetic functions. Cytotoxicity assays on PC12 cells, a cell line originated from a rat pheochromocytoma, an adrenal medulla tumor, demonstrated that Ag/AgO NPs synthesized with apolar (Ag/AgO NPs A) and polar (Ag/AgO NPs P) extracts exhibited significant cytotoxic effects, primarily driven by Ag ion release and the disruption of mitochondrial function.
View Article and Find Full Text PDFBiosensors (Basel)
December 2024
Fujian Key Laboratory of Translational Research in Cancer and Neurodegenerative Diseases, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China.
Simultaneous monitoring of antimicrobial responses to bacterial metabolic activity and biofilm formation is critical for efficient screening of new anti-biofilm drugs. A microbial fuel cell-based biosensor using as an electricigen was constructed. The effects of silver nanoparticles (AgNPs) on the cellular metabolic activity and biofilm formation of in the biosensors were investigated and compared with the traditional biofilm detection method.
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