Topical application and quantification of targeted, surface-enhanced Raman scattering (SERS) nanoparticles offer a new technique that has the potential for early detection of epithelial cancers of hollow organs. Although less toxic than intravenous delivery, the additional washing required to remove unbound nanoparticles cannot necessarily eliminate nonspecific pooling. Therefore, we developed a real-time, ratiometric imaging technique to determine the relative concentrations of at least two spectrally unique nanoparticle types, where one serves as a nontargeted control. This approach improves the specific detection of bound, targeted nanoparticles by adjusting for working distance and for any nonspecific accumulation following washing. We engineered hardware and software to acquire SERS signals and ratios in real time and display them via a graphical user interface. We report quantitative, ratiometric imaging with nanoparticles at pM and sub-pM concentrations and at varying working distances, up to 50 mm. Additionally, we discuss optimization of a Raman endoscope by evaluating the effects of lens material and fiber coating on background noise, and theoretically modeling and simulating collection efficiency at various working distances. This work will enable the development of a clinically translatable, noncontact Raman endoscope capable of rapidly scanning large, topographically complex tissue surfaces for small and otherwise hard to detect lesions.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763230PMC
http://dx.doi.org/10.1117/1.JBO.18.9.096008DOI Listing

Publication Analysis

Top Keywords

ratiometric imaging
12
raman endoscope
12
real-time ratiometric
8
surface-enhanced raman
8
raman scattering
8
clinically translatable
8
working distances
8
raman
5
nanoparticles
5
high-sensitivity real-time
4

Similar Publications

Development of a novel ICT-ESIPT-based NIR ratiometric fluorescent probe for specific detection of Hg in the environment and living organisms.

Spectrochim Acta A Mol Biomol Spectrosc

December 2024

School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China. Electronic address:

As a heavy metal contaminant, mercury ion (Hg) has caused great harm to environment and life. Mercury ions will migrate and transform in the environment and eventually accumulate in the human body, thus causing human poisoning. Therefore, it is of great significance to detect Hg in the environment and living bodies.

View Article and Find Full Text PDF

Zero-Crosstalk Tumor-Targeting Ratiometric Near-Infrared γ-Glutamyltranspeptidase Probe for Fluorescent-Guided Surgical Resection of Orthotopic Hepatic Tumor.

Anal Chem

January 2025

Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Hunan Provincial University Key Laboratory for Environmental and Ecological Health, College of Chemistry, Xiangtan University, Xiangtan 411105, P.R. China.

The challenge of "false positive" signals significantly complicates tumor localization and surgical resection, which are pivotal for successful tumor surgeries. Therefore, the development of a method for preoperative tumor localization and intraoperative margin determination holds considerable promise for improving surgical outcomes. In this study, a zero-crosstalk ratiometric tumor-targeting near-infrared (NIR) fluorescent probe was developed for precise cancer diagnosis and intraoperative navigation via NIR fluorescence imaging.

View Article and Find Full Text PDF

RpH-ILV: Probe for lysosomal pH and acute LLOMe-induced membrane permeabilization in cell lines and .

Sci Adv

January 2025

Department of Biochemistry Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.

Lysosomal pH dysregulation is a critical element of the pathophysiology of neurodegenerative diseases, cancers, and lysosomal storage disorders (LSDs). To study the role of lysosomes in pathophysiology, probes to analyze lysosomal size, positioning, and pH are indispensable tools. Here, we developed and characterized a ratiometric genetically encoded lysosomal pH probe, RpH-ILV, targeted to a subpopulation of lysosomal intraluminal vesicles.

View Article and Find Full Text PDF

Facile preparation of a hydrophilic Eu-based ratiometric fluorescent nanosensor for Cu ion detection and imaging in living cells.

Anal Methods

January 2025

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430072, China.

In this work, a hydrophilic Eu-based ratiometric fluorescent nanosensor (PAAC-Eu) was developed for Cu ion detection in aqueous solutions and imaging in living cells. The sensor was prepared a simple one-step reaction at room temperature, leveraging the synergistic coordination of commercially accessible polyacrylic acid (PAA) and coumarin-3-carboxylic acid (CCAH) with Eu ions. PAAC-Eu was easy to disperse in aqueous media and exhibited two characteristic emission bands at 406 nm and 618 nm, respectively, upon excitation at 350 nm.

View Article and Find Full Text PDF

Significance: Cellular metabolism is highly dynamic and strongly influenced by its local vascular microenvironment, gaining a systems-level view of cell metabolism is essential in understanding many critical biomedical problems in a broad range of disciplines. However, very few existing metabolic tools can quantify the major metabolic and vascular parameters together in biological tissues with easy access.

Aim: We aim to fill the technical gap by demonstrating a point-of-care, easy-to-use, easy-to-access, rapid, systematic optical spectroscopy platform for metabolic and vascular characterizations on biological models to enable scientific discoveries to translate more efficiently to clinical interventions.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!