AI Article Synopsis
Article Abstract
Globozoospermia, characterised by the presence of round spermatozoa lacking acrosomes in an ejaculate, is a known cause of male infertility. Semen analysis, including sperm chromatin structure assay, toluidine blue, chromomycin A3 and aniline blue staining and fluorescence in situ hybridisation, was performed in an infertile globozoospermic patient to establish to which extent these genetic factors contributed to his infertility. No spermatozoa capable of hyaluronan (HA) binding were detected in the HA binding assay. Increased rates of immature spermatozoa with defective replacement of histones by protamines, DNA breaks and disturbed chromatin integrity and sperm aneuploid for the sex chromosomes were observed. Intracytoplasmic sperm injection (ICSI) was used in three in vitro fertilisation (IVF) cycles, and enough morphologically well-developing embryos were obtained in each cycle. However, no pregnancy was achieved. The infertility of our couple, resistant to IVF/ICSI treatment, was most probably caused by a combination of male and female factors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/and.12156 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Post-testicular spermatozoa of a marine teleost can conduct cytoplasmic and mitochondrial translation.
iScience
January 2025
Institute of Marine Sciences, Spanish National Research Council (CSIC), 08003 Barcelona, Spain.
Translational silence of spermatozoa has long been considered the norm in animals. However, studies in mammals have shown that the mitochondrial ribosomal machinery is selectively activated during capacitation in the female reproductive tract, while cytosolic ribosomes remain inactive. Here, using quantitative proteomics in a piscine model species, we show that proteins involved in mRNA processing and cytoplasmic translation are predominantly accumulated in immature spermatozoa within the extratesticular excurrent ducts, while those related to flagellar motility are enriched in ejaculated (mature) sperm.
View Article and Find Full Text PDFThe Role of Melatonin on Caprine () Sperm Freezability: A Review.
Antioxidants (Basel)
November 2024
Laboratorio de Reproducción Animal, Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de Mexico, Cuautitlán Izcalli 54714, Mexico.
In mammals, the pineal hormone melatonin is the most powerful pacemaker of the master circadian clock and is responsible for reproduction in seasonal breeders. It is also well known that melatonin and its metabolites play antioxidant roles in many tissues, including reproductive cells. Melatonin synthesis and secretion from the pineal gland occurs during scotophase (the dark phase during a day-night cycle), while its inhibition is observed during photophase (period of light during a day-night cycle).
View Article and Find Full Text PDFClinical and Laboratorial Evaluation of Male Infertility. A Detailed Practical Approach.
Arch Med Res
December 2024
Department of Surgery, Division of Urology, Hospital das Clinicas, University of São Paulo Medical School, São Paulo, Brazil; Androscience, Science and Innovation Center in Andrology and High-Complex Clinical and Research Andrology Laboratory and The Androscience Institute for Science, Education and Advanced Projects in Male Health, São Paulo, Brazil; Men's Health Study Group, Institute for Advanced Studies, University of Sao Paulo, São Paulo, Brazil; Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. Electronic address:
Infertility, defined as the inability to achieve pregnancy after one year of unprotected, frequent sexual intercourse, is a global burden that affects approximately 15% of couples, or 190-230 million people worldwide, who are trying to start a family. The male contributes significantly, directly accounting for 30-35% and up to 52% of total couple infertility, affecting approximately 7-8% of all men. This work aims to present, in a didactic and objective way, a standardization of the initial steps toward a thorough evaluation of subfertile and infertile men.
View Article and Find Full Text PDFEvaluation of circANKLE2 & circL3MBTL4 -RNAs Expression in Fertile and Infertile Men.
Biochem Genet
November 2024
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt.
There are many factors that affect male fertility such as chronic health problems, psychological factors, and illnesses. Male infertility can be caused abnormal sperm function, low sperm production or even blockages that prevent the delivery of sperm. The aim of the work is to determine the expression pattern of the circularANKLE2 and circularL3MBTL4 RNA in spermatozoa from fertile and infertile males, as well as the relationship between these circRNA transcripts and sperm quality.
View Article and Find Full Text PDFSeasonality analysis on cryopreserved doses from the autochthonous cattle breeds Asturiana de la Montaña and Asturiana de los Valles.
Reprod Domest Anim
October 2024
Selección y Reproducción Animal-SERIDA, Gijón, Spain.
Germplasm banking is a fundamental tool for the preservation of autochthonous breeds. Semen cryopreservation is effective for this task, but protocols are adapted to commercial species, and post-thawing sperm quality could be sensitive to environmental cues. We compared the post-thawing sperm quality in doses from the CBA-SERIDA bank in northern Spain for the Asturiana de la Montaña (AM) and Asturiana de los Valles (AV) autochthonous cattle breeds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!