A humidity-sensitive hydrogel-Bacillus spore composite for micropatterning of biomolecular gradients.

Rev Sci Instrum

Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.

Published: August 2013

A composite material consisting of Bacillus subtilis spores suspended in a humidity sensitive hydrogel can be used to pattern biomolecules in different concentrations directly onto glass surfaces using a mechanical micromanipulator. By altering the relative humidity surrounding the composite gel during deposition, surface concentration of patterned biomolecules can be controlled and varied to create user-defined, biomolecular surface concentrations.

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Source
http://dx.doi.org/10.1063/1.4817971DOI Listing

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