AI Article Synopsis
- The genome of Gram-negative Aeromonas hydrophila 4AK4, used for industrial production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), was sequenced and revealed a chromosome size of 4,527,993 bp with 4,272 genes.
- Comparative analysis showed similarities to A. hydrophila ATCC 7966(T), known for its pathogenicity, but strain 4AK4 lacked the cytotonic enterotoxin (Ast), suggesting reduced virulence.
- The genomic data provides insights for enhancing polyhydroxyalkanoate (PHA) production through metabolic engineering of A. hydrophila 4AK4.
Article Abstract
The complete genome of Gram-negative Aeromonas hydrophila 4AK4 that has been used for industrial production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) was sequenced and annotated. Its chromosome is 4,527,993 bp in size encoding 4,272 genes, including 28 rRNA genes and 104 tRNA genes. Comparative analysis indicated that genome of A. hydrophila 4AK4 was similar to that of the A. hydrophila ATCC 7966(T), an intensively studied aeromonad for its pathogenicity related to its genomic information. Genes possibly coming from other species or even other genus were identified in A. hydrophila 4AK4. A large number of putative virulent genes were predicted. However, a cytotonic enterotoxin (Ast) is absent in A. hydrophila 4AK4, allowing the industrial strain to be different from other A. hydrophila strains, indicating possible reduced virulence of strain 4AK4, which is very important for industrial fermentation. Genes involved in polyhydroxyalkanoate (PHA) metabolism were predicted and analyzed. The resulting genomic information is useful for improved production of PHA via metabolic engineering of A. hydrophila 4AK4.
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Article Synopsis
- The genome of Gram-negative Aeromonas hydrophila 4AK4, used for industrial production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), was sequenced and revealed a chromosome size of 4,527,993 bp with 4,272 genes.
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[Application of high efficiency promoters in microbial production of 4-hydroxybutyric acid].
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School of Life Science, Tsinghua University, Beijing 100084, China.
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