AI Article Synopsis
- Vα14 iNKT cells activated by alpha-galactosylceramide (GalCer) produce a significant amount of cytokines, particularly IFN-γ, when stimulated.
- GalCer pretreatment enhances the response of splenocytes to various TLR agonists, boosting IFN-γ mRNA expression, particularly in NK and CD8 T cells.
- The study demonstrates that the combined stimulation of GalCer and TLR agonists significantly increases IFN-γ production, with the process involving the activation of certain signaling pathways like IκB-α phosphorylation.
Article Abstract
Vα14 natural killer T (iNKT) cells activated by alpha-galactosylceramide (GalCer) secrete a large amount of cytokines. Toll-like receptors (TLRs) play a critical role in the innate immune responses via the recognition of pathological antigen. Previously we demonstrated that the iNKT cells activated by GalCer augmented LPS-induced NO production in peritoneal cells. In this study, we examined the effect of GalCer and TLR agonists by IFN-γ production from splenocytes. Splenocytes pretreated with GalCer induced TLR3, 4, 7/8, and 9 agonists in vitro, resulting in the enhancement of IFN-γ mRNA expression. In particular, IFN-γ stimulated by GalCer and LPS was increased in NK cells and CD8 T cells, and inhibited by a neutralizing anti-IL-12 antibody. Pretreatment with GalCer enhanced the phosphorylation of IκB-α induced by LPS stimulation. The present study showed that co-stimulation of GalCer and TLR agonists powerfully induced the production of IFN-γ from splenocytes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759050 | PMC |
| http://dx.doi.org/10.1038/srep02559 | DOI Listing |
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