AI Article Synopsis
- The study explores using femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples.
- A Ti:Sa laser emitting ultrashort 12 fs pulses at 800 nm wavelength was used, alongside a light-emitting diode for comparison.
- Findings reveal significant differences in how pinhole size affects spatial resolution in DLHM when using different light sources, uncovering previously unrecognized phenomena in the field.
Article Abstract
The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.
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| http://dx.doi.org/10.1364/OL.38.003205 | DOI Listing |
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