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Delivery of natural polyphenols by polymeric nanoparticles improves the resistance of endothelial progenitor cells to oxidative stress. | LitMetric

AI Article Synopsis

  • Bone marrow-derived endothelial progenitor cells (EPCs) play a key role in blood vessel repair and are negatively affected by oxidative stress, prompting this study's investigation into mucoadhesive polymeric nanoparticles as a protective delivery system for natural products.* -
  • The study assessed the antioxidant capacities and total polyphenol contents of red grape seed extracts (GSE), revealing that both pre-veraison and ripe extracts have strong antioxidant properties, with the pre-veraison extract containing higher polyphenol levels.* -
  • Results indicated that the nanoparticles (NPs) effectively encapsulated GSE and enhanced cell viability against oxidative damage, highlighting the potential of nanotechnology in delivering nutraceuticals to safeguard EPCs and promote their survival.*

Article Abstract

Purpose: Bone marrow-derived endothelial progenitor cells (EPCs) circulate into peripheral blood and significantly contribute to neo-vascularisation and re-endothelialisation as part of the process of vascular repair. Several studies have reported decreased EPC number in the presence of oxidative stress. Aim of this study was to evaluate the validity of mucoadhesive polymeric nanoparticles as a delivery system of natural products able to protect EPCs from oxidative stress.

Methods: The total polyphenol content and antioxidant capacity of red grape seed extract (GSE) either pre-veraison (p-GSE) or ripe (r-GSE) were measured. Cell viability was evaluated by WST-1 assay. Nanoparticles were prepared by ionotropic crosslinking of two structurally different thiolated quaternary ammonium-chitosan conjugates. A hyaluronic acid solution, containing p-GSE or r-GSE, was added to a stirred solution of each of the two chitosan derivatives to obtain p- or r-GSE loaded nanoparticles (NP) of two types.

Results: Both GSE types demonstrated strong antioxidant capacity. p-GSE showed a higher content in total polyphenols compared to r-GSE. NP size was in the 310-340 nm range, with 24 h stability, and nearly 100% encapsulation efficiency for both GSE types. NP were internalized by cells to an extent related directly with their surface charge intensity. GSE-NP uptake significantly improved cell viability and resistance to oxidation.

Conclusions: Nanotechnology has a great potential in nutraceutical delivery. The present results suggest that NP is a highly promising polyphenol carrier system particularly useful to protect EPCs from oxidative stress, thus improving their survival.

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Source
http://dx.doi.org/10.1016/j.ejps.2013.08.008DOI Listing

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