Ganoderma applanatum is one of the most popular medicinal mushrooms due to the various biologically active components it produces. This study was conducted to obtain basic information regarding the mycelial culture conditions of Ganoderma applanatum. Based on the colony diameter and mycelial density, PDA, YMA and MCM media were suitable for the mycelial growth of the mushroom. The optimum temperature for mycelial growth was found to be 25~30℃. The optimum carbon and nitrogen sources were mannose and dextrin, respectively, and the optimum C/N ratio was 2 to 10 when 2% glucose was used. Other minor components required for the optimal growth included thiamine-HCl and biotin as vitamins, succinic acid and lactic acid as organic acids, and MgSO4·7H2O, KH2PO4 and NaCl as mineral salts.
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http://dx.doi.org/10.4489/MYCO.2009.37.2.094 | DOI Listing |
Sci Rep
January 2025
College of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, Lanzhou, 730070, China.
Recently, a new bacterial disease was detected on cucumber stalks. In order to study the pathogenesis of this disease, the pathogenic bacteria were isolated and identified on the basis of morphological and molecular characteristics, and further analyzed for pathogenicity and antagonistic evaluation. Pathogenicity analysis showed that HlJ-3 caused melting decay and cracking in cucumber stems, and the strain reisolated from re-infected cucumber stalks was morphologically identical to HlJ-3 colonies, which is consistent with the Koch's postulates.
View Article and Find Full Text PDFPlant Dis
January 2025
Tennessee State University, Otis Floyd Nursery Research Center, 472 Cadillac Lane, McMinnville, Tennessee, United States, 37110;
Incense cedar [ (Torr.) Florin] is a coniferous evergreen tree, indigenous to western North America, that is being evaluated in Tennessee for its adaptability to eastern U.S.
View Article and Find Full Text PDFPlant Dis
January 2025
Guangdong Academy of Agricultural Sciences, Crop Research Institute, Wushan Road, Tianhe District, guangzhou, China, 510640;
Sweet potato ( (L.) Lam) is a major food crop that is cultivated in southern China (Huang et al. 2020).
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address:
Appl Microbiol Biotechnol
December 2024
Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kitashirakawaoiwakecho, Kyoto, 606-8502, Japan.
Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-assisted genome editing has been applied to several major edible agaricomycetes, enabling efficient gene targeting. This method is promising for rapid and efficient breeding to isolate high-value cultivars and overcome cultivation challenges. However, the integration of foreign DNA fragments during this process raises concerns regarding genetically modified organisms (GMOs) and their regulatory restrictions.
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