Due to contaminant Escherichia coli DNA present in recombinant Taq polymerase reagents, it is not possible to reliably detect low levels of E. coli in samples using the quantitative polymerase chain reaction (qPCR) assay. Native Taq polymerase was successfully used in this study to detect five uidA gene copies (5 fg of genomic DNA) of the uidA gene.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2166/wh.2013.201 | DOI Listing |
Publication Analysis
Top Keywords
uida gene
12
qpcr assay
8
escherichia coli
8
taq polymerase
8
eliminating false
4
false positives
4
positives qpcr
4
assay detection
4
detection uida
4
gene escherichia
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!