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The rapid detection of Cryptosporidium and Giardia species in clinical stools using the Quik Chek immunoassay. | LitMetric

The rapid detection of Cryptosporidium and Giardia species in clinical stools using the Quik Chek immunoassay.

Parasitol Int

Scottish Parasite Diagnostic and Reference Laboratory, Stobhill Hospital, Glasgow G21 3UW, United Kingdom. Electronic address:

Published: December 2013

AI Article Synopsis

  • The study compares the traditional microscopy method with the Quik Chek antigen immunoassay for detecting Cryptosporidium and Giardia in stool samples.
  • Results showed that Quik Chek has a sensitivity of 87.6% and specificity of 98.9% for Cryptosporidium, and sensitivity of 93.3% and specificity of 99.4% for Giardia.
  • The findings suggest that the Quik Chek assay is a reliable rapid test and supports its use in diagnostic microbiology labs.

Article Abstract

Diagnostic testing in the United Kingdom for Cryptosporidium and Giardia species is routinely performed by microscopy. In this study, two hundred stool samples from human clinical cases were examined for the presence of these two parasites comparing microscopy with an antigen immunoassay, Quik Chek (Techlab, Inc.). The Quik Chek assay was shown to have a sensitivity and specificity for Cryptosporidium detection of 87.6% and 98.9% respectively and for Giardia detection, 93.3% and 99.4% respectively. The high correlation with microscopy data provides evidence to support implementation of this rapid test within diagnostic microbiology laboratories.

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Source
http://dx.doi.org/10.1016/j.parint.2013.08.008DOI Listing

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