AI Article Synopsis
- Birds exhibit a more primitive form of epiblast development compared to eutherian mammals, making them a valuable model for studying mammalian epiblasts.
- The text outlines techniques for handling chick embryos, including methods for RNA extraction, gene expression analysis, and culture setups for testing molecular interactions.
- Additionally, it covers how to culture primary chicken epiblasts and create stable epiblast stem cell lines, as well as techniques for assessing their pluripotency.
Article Abstract
Compared to eutherian mammals, birds retain a primitive form of epiblast development. Molecular studies of the avian epiblast can provide valuable insight for mammalian epiblast research. Here, we introduce several basic techniques in handling epiblast-stage embryos of the chick, the major model organism for avian developmental biology studies. We describe how to collect embryos for RNA extraction and gene expression analysis, to set up ex ovo New culture for overexpression, bead graft and small molecule-based inhibitor studies, and to carry out whole-mount RNA in situ hybridization analysis. We introduce a novel and simple method for molecular perturbation of the epiblast differentiation in ovo. We also describe how to perform primary chicken epiblast cell culture, to establish stable epiblast stem cell (Epi-SC) lines, and to assay for pluripotency in primary epiblast cells and Epi-SCs.
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| http://dx.doi.org/10.1007/978-1-62703-628-3_12 | DOI Listing |
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