We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/nmeth.2610 | DOI Listing |
Publication Analysis
Top Keywords
dmso enhances
4
enhances electrospray
4
electrospray response
4
response boosting
4
boosting sensitivity
4
sensitivity proteomic
4
proteomic experiments
4
experiments report
4
report low
4
low percentages
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!