AI Article Synopsis
- The growing interest in personalized medicine is driving the need for innovative technologies to analyze clinical samples, particularly for cancer diagnosis and prognosis.
- A method using EpCAM-targeted buoyant microbubbles (MBs) has been developed to effectively capture and isolate tumor cells from various biological samples like blood and urine.
- This technique demonstrates over 90% binding efficiency with tumor cells and can isolate them in a short time, indicating its potential as a valuable tool for quickly enriching cancer biomarkers.
Article Abstract
With the emerging interest in personalized medicine, there is strong demand for new technologies for clinical sample interrogation. Exfoliated tumor cells in variety of pathological samples (e.g., blood, bone marrow, urine) could provide invaluable information for diagnosis and prognosis of cancers. Here we describe a detailed method for capture and isolation of tumor cells in medium, blood, or large issue buffy coat using EpCAM-targeted buoyant microbubbles (MBs). Perflorohexane gas lipid shell MBs were prepared with emulsification method and conjugated with antibody as described by us before [25]. The binding of EpCAM-targeted MBs to A549 (human lung carcinoma) and 4T1 (mouse breast carcinoma) cells spiked into BSA/PBS or blood was more than 90%, which was comparable with commercial anti-EpCAM immunomagnetic beads (DynaBeads). Anti-EpCAM MBs efficiently (75-82%) isolated BxPC3 pancreatic tumor cells spiked into medium, blood or a buffy coat, within 15-30 min of incubation. We discuss MB parameters and experimental conditions critical to achieve efficient cells binding and isolation. In conclusion, MB-assisted cell isolation is a promising method for rapid enrichment of cells and biomarkers from biological samples.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841068 | PMC |
| http://dx.doi.org/10.1016/j.ymeth.2013.08.008 | DOI Listing |
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