New application areas for chitin and its derivatives have been extensively investigated and there is a solid, growing demand for new chitin sources. In this present study, chitin content of Daphnia magna resting egg (18-21%) was determined for the first time. FTIR, elemental analysis, TGA, XRD and SEM studies revealed the structural and thermal properties of extracted α-chitin. This study suggests that D. magna resting eggs can be exploited as an attractive alternative chitin source.
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http://dx.doi.org/10.1016/j.ijbiomac.2013.08.016 | DOI Listing |
Animals (Basel)
September 2024
Department of Medical and Surgical Sciences, Magna Græcia University of Catanzaro, 88100 Catanzaro, Italy.
Currently, in dairy farming, there is growing concern for the welfare of calves during the critical period between the separation from their dams and weaning. During weaning, rationed feeding is a practice used to improve feed efficiency and control the calves' growth, but it could also have negative consequences associated with hunger and feed restriction behavior. One such consequence could be the performance by calves of stereotyped behaviors indicative of poor welfare, such as non-nutritive oral behaviors.
View Article and Find Full Text PDFJ Neurosci Methods
October 2024
Laboratory of General Physiology, Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia 27100, Italy.
Background: The conventional "whole-cell patch-clamp" recording technique is widely used to measure the resting membrane potential (V) and to dissect the underlying membrane ionic conductances in isolated vascular endothelial cells.
New Method: Herein, we assessed whether the automated patch-clamp (APC) technology, which replaces the traditional patch-pipette with a planar substrate to permit researchers lacking formal training in electrophysiology to generate large amounts of data in a relatively short time, can be used to characterize the bioelectrical activity of vascular endothelial cells. We assessed whether the Port-a-Patch planar patch-clamp system, which is regarded as the smallest electrophysiological rig available on the market, can be used to measure the V and resting membrane currents in the human cerebrovascular endothelial cell line, hCMEC/D3.
Front Neurol
May 2024
Neuroscience Research Center, University "Magna Graecia", Catanzaro, Italy.
Introduction: Distinguishing tremor-dominant Parkinson's disease (tPD) from essential tremor with rest tremor (rET) can be challenging and often requires dopamine imaging. This study aimed to differentiate between these two diseases through a machine learning (ML) approach based on rest tremor (RT) electrophysiological features and structural MRI data.
Methods: We enrolled 72 patients including 40 tPD patients and 32 rET patients, and 45 control subjects (HC).
Brain
July 2024
Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, 81377 Munich, Germany.
Knee osteoarthritis is the most prevalent type of osteoarthritis. Patients frequently encounter pain triggered by movement that evolves into impaired joint function. Needing persistent rest or having night-time pain signifies advanced disease.
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