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Alantolactone induces apoptosis in RKO cells through the generation of reactive oxygen species and the mitochondrial pathway. | LitMetric

Alantolactone induces apoptosis in RKO cells through the generation of reactive oxygen species and the mitochondrial pathway.

Mol Med Rep

National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, Jilin 130024, P.R. China.

Published: October 2013

AI Article Synopsis

  • Alantolactone, derived from Inula helenium, shows promising anticancer effects in colorectal cancer cells, specifically RKO human colon cancer cells.
  • The study found that alantolactone reduces cell viability and promotes apoptosis in these cells through the accumulation of reactive oxygen species (ROS) and mitochondrial disruption.
  • The mechanism involves downregulating anti-apoptotic protein Bcl-2, boosting pro-apoptotic protein Bax, and activating caspases, suggesting that alantolactone could be a potential treatment for human colon cancer.

Article Abstract

Alantolactone, a methanol extract of Inula helenium, possesses anticancer properties in a number of cancer cell lines. However, its anticancer effect on human colorectal cancer cells and the underlying mechanisms remain to be elucidated. In the present study, the effects of alantolactone on cell viability and apoptosis in RKO human colon cancer cells were investigated. Alantolactone treatment of RKO cells was found to result in dose‑dependent inhibition of cell viability and induction of apoptosis, accompanied with the accumulation of reactive oxygen species (ROS) and the disruption of mitochondrial membrane potential. In addition, these effects were blocked with N‑acetylcysteine, a specific ROS inhibitor. Western blotting indicated that exposure of RKO cells to alantolactone is associated with the downregulation of Bcl‑2, induction of Bax and activation of caspase‑3 and ‑9. These results indicated that a ROS‑mediated mitochondria‑dependent pathway is involved in alantolactone‑induced apoptosis. From these observations, it was hypothesized that alantolactone may be used for the treatment of human colon cancer.

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Source
http://dx.doi.org/10.3892/mmr.2013.1640DOI Listing

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