Efficient in vivo depletion of CD8(+) T lymphocytes in common marmosets by novel CD8 monoclonal antibody administration.

Immunol Lett

Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Hachimandai, Tsukuba, Ibaraki 305-0843, Japan; Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan. Electronic address:

Published: July 2014

In order to directly demonstrate the roles of CD8(+) T lymphocytes in non-human primates, in vivo depletion of the CD8(+) T cells by administration of a CD8-specific monoclonal antibody (mAb) is one of the crucial techniques. Recently, the common marmoset (Callithrix jacchus), which is classified as a New World monkey, has been shown useful as an experimental animal model for various human diseases such as multiple sclerosis, Parkinson's disease and a number of infectious diseases. Here we show that an anti-marmoset CD8 mAb 6F10, which we have recently established, efficiently depletes the marmoset CD8(+) T lymphocytes in vivo, i.e., the administration of 6F10 induces drastic and specific reduction in the ratio of the CD8(+) T cell subset for at least three weeks or longer. Our finding will help understand the pivotal role of CD8(+) T cells in vivo in the control of human diseases.

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http://dx.doi.org/10.1016/j.imlet.2013.08.005DOI Listing

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