AI Article Synopsis
- A study evaluated the effectiveness of direct molecular testing (PCR-ESI-MS) on whole blood to identify bacteria and yeasts in patients suspected of bloodstream infections, involving 464 positive and 442 negative blood culture patients.
- The combination of PCR-ESI-MS and blood culture showed a 78.6% agreement in identifying pathogens, with a significant proportion of culture-negative cases being confirmed as true infections.
- This method accelerates pathogen detection and improves diagnostic accuracy for septicemia when used alongside traditional blood cultures.
Article Abstract
A prospective study was performed to determine the value of direct molecular testing of whole blood for detecting the presence of culturable and unculturable bacteria and yeasts in patients with suspected bloodstream infections. A total of 464 adult and pediatric patients with positive blood cultures matched with 442 patients with negative blood cultures collected during the same period were recruited during a 10-month study. PCR amplification coupled with electrospray ionization mass spectrometry (PCR-ESI-MS) plus blood culture reached an overall agreement of 78.6% in the detection and species-level identification of bacterial and candidal pathogens. Of 33 culture-negative/PCR-ESI-MS-positive specimens, 31 (93.9%) were judged to be truly bacteremic and/or candidemic based on a medical chart review and analytical metrics. Among the 15 culture-positive specimens in which PCR-ESI-MS detected additional bacterial or yeast species, 66.7% and 20.0% of the additional positive specimens by PCR-ESI-MS were judged to be truly or possibly bacteremic and/or candidemic, respectively. Direct analysis of blood samples by PCR-ESI-MS rapidly detects bacterial and yeast pathogens in patients with bloodstream infections. When used in conjunction with blood culture, PCR-ESI-MS enhances the diagnostics of septicemia by shortening test turnaround time and improving yields.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3889730 | PMC |
| http://dx.doi.org/10.1128/JCM.00876-13 | DOI Listing |
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