AI Article Synopsis
- The Rift Valley fever virus (RVFV) has multiple structural proteins, including nucleoprotein (N) and glycoproteins (Gn and Gc), as well as nonstructural proteins (NSm and NSs).
- Researchers used a baculovirus system to express these proteins and confirmed their presence through western blot analysis, showing that the glycoproteins were glycosylated.
- The study highlights the potential of these recombinant proteins for diagnostic assays, particularly in differentiating between infected and vaccinated animals, enhancing disease management strategies.
Article Abstract
The Rift Valley fever virus (RVFV) encodes the structural proteins nucleoprotein (N), aminoterminal glycoprotein (Gn), carboxyterminal glycoprotein (Gc), and L protein, 78-kD, and the nonstructural proteins NSm and NSs. Using the baculovirus system, we expressed the full-length coding sequence of N, NSs, NSm, Gc, and the ectodomain of the coding sequence of the Gn glycoprotein derived from the virulent strain of RVFV ZH548. Western blot analysis using anti-His antibodies and monoclonal antibodies against Gn and N confirmed expression of the recombinant proteins, and in vitro biochemical analysis showed that the two glycoproteins, Gn and Gc, were expressed in glycosylated form. Immunoreactivity profiles of the recombinant proteins in western blot and in indirect enzyme-linked immunosorbent assay against a panel of antisera obtained from vaccinated or wild type (RVFV)-challenged sheep confirmed the results obtained with anti-His antibodies and demonstrated the suitability of the baculo-expressed antigens for diagnostic assays. In addition, these recombinant proteins could be valuable for the development of diagnostic methods that differentiate infected from vaccinated animals (DIVA).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777644 | PMC |
| http://dx.doi.org/10.1089/vbz.2012.1285 | DOI Listing |
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