AI Article Synopsis
- Understanding how molecules attach to human serum albumin (HSA) is crucial for anticipating potential drug interactions.
- We discovered a new fluorescent dye called BD140 that shows increased fluorescence and binds specifically to site 2 on HSA.
- By mixing BD140 with dansylamide, we created a fluorescent dye combination that allows for efficient testing of how various drugs interact with HSA's binding sites.
Article Abstract
Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.
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| http://dx.doi.org/10.1021/co400060b | DOI Listing |
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