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Constructing phylogenies in the presence of intra-individual site polymorphisms (2ISPs) with a focus on the nuclear ribosomal cistron. | LitMetric

Constructing phylogenies in the presence of intra-individual site polymorphisms (2ISPs) with a focus on the nuclear ribosomal cistron.

Syst Biol

Bolus Herbarium, Department of Biological Sciences, University of Cape Town, Cape Town, Western Cape, 7700, South Africa; and Department of Palaeobiology, Swedish Museum of Natural History, P.O. Box 50007, 104 05 Stockholm, Sweden.

Published: January 2014

AI Article Synopsis

  • Nuclear DNA is essential for studying evolutionary relationships, but variations within an individual's genes, known as Intra-Individual Site Polymorphisms (2ISPs), complicate the analysis.
  • Traditional phylogenetic methods often overlook these variations, leading to potential inaccuracies in reconstructions.
  • The study proposes treating 2ISPs as informative characters, showing that this approach improves phylogenetic inference across multiple common methods, particularly in scenarios involving nuclear DNA with polymorphisms.

Article Abstract

Nuclear DNA is widely used to estimate phylogenetic and phylogeographic relationships. Nuclear gene variants may be present in an individual's genome, and these result in Intra-Individual Site Polymorphisms (2ISP; pronounced "twisp") in direct-PCR or individual-consensus sequences based on a sample of clones or fragment sequences from next generation sequencing (NGS). 2ISPs can occur fairly often, especially within, but not restricted to, high-copy-number regions such as the widely used internal transcribed spacers of the nuclear ribosomal cistron. Dealing with 2ISPs has been problematic as phylogeny reconstruction optimality criteria generally do not take account of this variation. Here we test whether an approach that treats 2ISPs as additional (termed "informative"), rather than ambiguous, characters offers improved support in three common criteria used for phylogenetic inference: Minimum Evolution (via Neighbour Joining), Maximum Parsimony, and Maximum Likelihood. We demonstrate significant improvements using the 2ISP-informative treatment with simulated, real-world, and case-study data sets. We envisage that this 2ISP-informative approach will greatly aid phylogenetic inference using any nuclear DNA regions that contain polymorphisms within individuals (including consensus sequences generated from NGS), especially at the intrageneric or intraspecific level.

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Source
http://dx.doi.org/10.1093/sysbio/syt052DOI Listing

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