Aminopeptidase N (APN) is known to play important roles in tumor angiogenesis, tumor cell invasion, and metastasis. Thus, APN is an attractive biomarker for imaging tumor angiogenesis. Here we report results obtained from biodistribution and single photon emission computed tomography (SPECT) imaging studies of a technetium-99m labeled probestin (a potent APN inhibitor) conjugate containing a tripeptide, Asp-DAP-Cys (DAP=2,3-diaminopropionic acid), chelator and a 8-amino-3,6-dioxaoctanoic acid (PEG2) linker conducted in nude mice xenografted with HT-1080 human fibrosarcoma tumors (APN-positive tumors). These results collectively demonstrate that (99m)Tc-probestin uptake by tumors and other APN expressing tissues in vivo is specific and validate the use of probestin as a vector for targeting APN in vivo.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bmcl.2013.07.046 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Circulating levels of adiponectin and AdipoR expression in peripheral blood mononuclear cells are associated with lower respiratory tract Infection.
Front Immunol
January 2025
Department of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Objective: The role of adiponectin (APN) in regulating inflammation is well recognized in metabolic disease, but the dysregulation of APN in lower respiratory tract infection (LRTI) remains controversial. We aimed to measure APN and its signaling receptors, adiponectin receptor (AdipoR), in peripheral blood mononuclear cells (PBMCs) from LRTI patients to explore their potential roles in the LRTI process.
Methods: A total of 99 LRTI patients from the Second Xiangya Hospital of Central South University were categorized into acute (n=35) and non-acute (n=64), and non-severe (n=62) and severe (n=37) groups.
The Expression of Proteases and the Oligopeptide Transporter in the Yolk Sac Membrane, Proventriculus, and Small Intestine During the Development of Embryo.
Biology (Basel)
November 2024
Department of Biology and Biotechnology, Faculty of Science, Hashemite University, P.O. Box 330127, Zarqa 13133, Jordan.
The role of the yolk sac membrane (YSM) and digestive tract in the processing of egg yolk proteins during embryogenesis is unexplored in the duck . Here, we investigated in the duck embryo the function of the YSM, proventriculus, and small intestine in protein digestion and uptake. We tested the expression of aminopeptidase N () and the oligopeptide transporter as well as the expression of cathepsin B () and cathepsin D () lysosomal genes in the YSM during incubation days 12, 14, 16-18, 20, 22, 24, 26, and 28 (the day of hatch).
View Article and Find Full Text PDFAdiponectin deficiency prevents chronic colitis-associated colonic fibrosis via inhibiting CXCL13 production.
J Adv Res
December 2024
Hebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004, China. Electronic address:
Introduction: Colonic fibrosis is a long-term complication of inflammatory bowel disease (IBD), often leading to functional impairment, intestinal obstruction, and surgery. Adiponectin (APN) is an adipokine derived from adipocytes that plays a pleiotropic role in fibrosis regulation, depending on tissue and cell type specific or disease context, but its role in colonic fibrosis remains unclear.
Objective: To explore the role and involved mechanism of APN in chronic colitis-associated colonic fibrosis.
The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch.
Poult Sci
December 2024
Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Australia. Electronic address:
The early post-hatching phase remains to be one of the most vulnerable phases in broiler production. Some essential oils have been reported to improve gut health and growth in broiler chickens when applied to post-hatching diets. However, in-feed applications are unable to prevent the health challenges observed immediately after hatching.
View Article and Find Full Text PDFRelationship of adiponectin, leptin and reproductive endocrine hormones with insulin resistance in polycystic ovary syndrome patients with normal BMI.
Gynecol Endocrinol
December 2024
Department of Gynaecology and Obstetrics, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Objective: This study aimed to analyze the correlation of adiponectin (APN) and leptin (LEP), reproductive endocrine hormones with insulin resistance (IR) in polycystic ovary syndrome (PCOS) patients with normal body mass index (BMI).
Methods: Totally 160 PCOS patients were categorized into the Normal BMI (BMI: 18.5-25 kg/m, = 62) and High BMI (BMI > 25 kg/m, = 98) groups.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!