Cells have a wide range of capacities to remove extracellular hydrogen peroxide. At higher concentrations of extracellular HO (micromolar) the rate of removal can be approximated by a rate equation that is first-order in the concentration of HO and cell density. Here we present a method to determine the observed rate constant for the removal of extracellular HO on a per cell basis. In the cells examined, when exposed to 20 μM HO, these rate constants () range from 0.46 × 10 s cell L for Mia PaCa-2 cells (human pancreatic carcinoma) to 10.4 × 10 s cell L for U937 cells (human histiocytic lymphoma). For the relatively small red blood cell = 2.9 × 10 s cell L. These rate constants, , can be used to compare the capacity of cells to remove higher levels of extracellular HO, as often presented in cell culture experiments. They also provide a means to estimate the rate of removal of extracellular HO, rate = - [HO] (cells L), and the half-life of a bolus of HO. This information is essential to optimize experimental design and interpret data from experiments that expose cells to extracellular HO.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3736862 | PMC |
| http://dx.doi.org/10.1016/j.redox.2013.01.011 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nisin A Treatment to Protect Honey Bee Larvae from European Foulbrood Disease.
Probiotics Antimicrob Proteins
January 2025
Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya, Tokyo, 156-8502, Japan.
European foulbrood (EFB) is a bee larvae-specific infectious disease and the causative pathogen is Melissococcus plutonius. Broad-spectrum antibiotics have classically been used in many countries to control the pathogens; however, their use in apiaries was discontinued in several countries due to concerns regarding the health of bees and humans. Therefore, the development of alternative treatments for use in apiaries that are safe for bees and humans is essential.
View Article and Find Full Text PDFToxicological problems of tattoo removal: characterization of femtosecond laser-induced fragments of Pigment Green 7 and Green Concentrate tattoo ink.
Arch Toxicol
January 2025
STARTNETICS - Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133, Rome, Italy.
Femtosecond lasers represent a novel tool for tattoo removal as sources that can be operated at high power, potentially leading to different removal pathways and products. Consequently, the potential toxicity of its application also needs to be evaluated. In this framework, we present a comparative study of Ti:Sapphire femtosecond laser irradiation, as a function of laser power and exposure time, on water dispersions of Pigment Green 7 (PG7) and the green tattoo ink Green Concentrate (GC), which contains PG7 as its coloring agent.
View Article and Find Full Text PDFDevelopment of a novel laboratory approach for evaluating the adhesion strength of biofilms to human dentine.
Int Endod J
January 2025
School of Medicine and Dentistry, Griffith University, Gold Coast, Australia.
Introduction: Biofilms may show varying adherence strengths to dentine. This study quantified the shear force required for the detachment of multispecies biofilm from the dentine using fluid dynamic gauging (FDG) and computation fluid dynamics (CFD). To date this force has not been quantified.
View Article and Find Full Text PDFOptimizing Closed Approach Preservation Rhinoplasty by Ultrasonic-Assisted Techniques for Enhanced Precision.
Aesthet Surg J
January 2025
Careggi University Hospital, Florence, Italy.
Background: Preservation rhinoplasty, particularly dorsal preservation, has gained prominence with the advent of piezoelectric instruments, which minimize trauma and enhance precision in bone reshaping. This approach presents unique challenges, especially in closed techniques, where spatial limitations complicate the use of piezotomes.
Objectives: To evaluate outcomes of piezo-assisted preservation rhinoplasty using specialized instrumentation to enhance visibility, precision, and postoperative recovery in closed rhinoplasty procedures.
Site-Specific for CO Photoreduction with Single-Atom Ni on Strained TiO Derived from Bimetallic Metal-Organic Frameworks.
Small
January 2025
College of Materials Science and Engineering, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China.
The photocatalytic reduction of CO in water to produce fuels and chemicals is promising while challenging. However, many photocatalysts for accomplishing such challenging task usually suffer from unspecific catalytic active sites and the inefficient charge carrier's separation. Here, a site-specific single-atom Ni/TiO catalyst is reported by in situ topological transformation of Ni-Ti-EG bimetallic metal-organic frameworks.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!