AI Article Synopsis
- Peripheral arterial disease (PAD) is caused by plaque buildup in arteries, leading to issues in blood flow, and traditional cell therapies have struggled with low effectiveness due to poor cell retention.
- This study introduces a novel technique using transfer printing with thermosensitive hydrogels to deliver myoblast cell layers directly to ischemic hindlimb tissue, improving retention compared to traditional injections.
- Results showed that this method not only maintained cell structure and function but also led to better therapeutic outcomes in mice, indicating its potential as a new strategy for treating PAD.
Article Abstract
Peripheral arterial disease (PAD) is characterized by the altered structure and function of arteries caused by accumulated plaque. There have been many studies on treating this disease by the direct injection of various types of therapeutic cells, however, the low cell engraftment efficiency and diffusion of the transplanted cells have been major problems. In this study, we developed an approach (transfer printing) to deliver monolayer of cells to the hindlimb ischemic tissue using thermosensitive hydrogels, and investigated its efficacy in long term retention upon transplantation and therapeutic angiogenesis. We first investigated the in vitro maintenance of robust cell-cell contacts and stable expression of the ECM proteins in myoblast layer following transfer printing process. In order to confirm the therapeutic effect of the myoblasts in vivo, we cultured a monolayer of C2C12 myoblasts on thermosensitive hydrogels, which was then transferred to the hindlimb ischemia tissue of athymic mice directly from the hydrogel by conformal contact. The transferred myoblast layer was retained for a longer period of time than an intramuscularly injected cell suspension. In addition, the morphology of the mice and laser Doppler perfusion (28 days after treatment) supported that the myoblast layer enhanced the therapeutic effects on the ischemic tissue. In summary, the transplantation of the C2C12 myoblast layer using a tissue transfer printing method could represent a new approach for the treatment of PAD by therapeutic angiogenesis.
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| http://dx.doi.org/10.1016/j.biomaterials.2013.07.071 | DOI Listing |
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