Weakly haemolytic anaerobic intestinal spirochaetes of the genus Brachyspira are commonly identified based on species-specific gene sequences. Apart from the pathogenic Brachyspira pilosicoli, the distribution and disease associations of the other weakly haemolytic Brachyspira species in pigs have not been comprehensively investigated. In this study weakly haemolytic Brachyspira isolates (n=67) from Spanish and Portuguese pigs with diarrhoea, negative in a routine diagnostic PCR for B. pilosicoli, were identified by sequencing their NADH oxidase genes (nox). Nearly half the isolates were identified as Brachyspira murdochii (n=31; 46.3%). The others were Brachyspira innocens (n=26; 38.8%), Brachyspira intermedia (n=7; 10.4%), "Brachyspira pulli" (n=1; 1.5%) and a potentially novel Brachyspira species (n=2; 3%). Multilocus sequence typing (MLST) on a subset of 18 isolates confirmed their species designations, including the potential new species, and identified similarities to strains from other countries.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.rvsc.2013.07.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Naturopathic Management of Hereditary Spherocytosis: A Case Report.
Altern Ther Health Med
November 2024
Hereditary spherocytosis (HS) is a common variant of inherited hemolytic anemia, in which abnormalities of red blood cells (RBC) structural proteins lead to loss of erythrocyte membrane surface area, resulting in spherical, hyperdense, weakly deformable RBCs. It is characterized by the presence of osmotically fragile erythrocytes, known as spherocytes in peripheral blood smear (PBS). The mutations that cause HS occur in genes encoding for red blood cell membrane/cytoskeletal proteins, predominantly, ankyrin-1, α- and β-spectrins, band-3, and protein 4.
View Article and Find Full Text PDFTowards efficient Ir(III) anticancer photodynamic therapy agents by extending π-conjugation on N^N ligands.
Dalton Trans
July 2024
Universidad de Burgos, Departamento de Química, Facultad de Ciencias, Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.
In this work we disclose a new family of biscyclometallated Ir(III) complexes of the general formula [Ir(C^N)(N^N)]Cl (IrL1-IrL5), where HC^N is 1-phenyl-β-carboline and N^N ligands (L1-L5) are different diimine ligands that differ from each other in the number of aromatic rings fused to the bipyridine scaffold. The photophysical properties of IrL1-IrL5 were thoroughly studied, and theoretical calculations were performed for a deeper comprehension of the respective variations along the series. All complexes exhibited high photostability under blue light irradiation.
View Article and Find Full Text PDFDimerization of the β-Hairpin Membrane-Active Cationic Antimicrobial Peptide Capitellacin from Marine Polychaeta: An NMR Structural and Thermodynamic Study.
Biomolecules
March 2024
M.M. Shemyakin & Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
Capitellacin is the β-hairpin membrane-active cationic antimicrobial peptide from the marine polychaeta . Capitellacin exhibits antibacterial activity, including against drug-resistant strains. To gain insight into the mechanism of capitellacin action, we investigated the structure of the peptide in the membrane-mimicking environment of dodecylphosphocholine (DPC) micelles using high-resolution NMR spectroscopy.
View Article and Find Full Text PDF[Serological Characteristics and Clinical Significance of Irregular Antibodies in Pregnant Women].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
February 2024
Department of Blood Transfusion, Women and Children's Hospital of Chongqing Medical University, Chongqing Health Center for Women and Children, Chongqing 401174, China.E-mail:
Article Synopsis
- The study aimed to analyze the presence of irregular antibodies in pregnant women and their clinical implications.
- The screening of 151,471 pregnant women showed a 0.91% positive rate for irregular antibodies, with higher rates found in the third trimester compared to earlier trimesters.
- The majority of positive results were weakly positive, and women who were on their second or more pregnancies had a higher prevalence of irregular antibodies.
Asymmetric disturbance and permeabilization of bilayer membranes by 3-nm carbon dots.
J Hazard Mater
March 2024
Songshan Lake Materials Laboratory, Dongguan 523808, Guangdong, China. Electronic address:
Small-sized fluorescent carbon dots (CDs) are gaining increasing attention in the field of biomedical applications. The environmental and biological compatibility of positively charged CDs has been extensively investigated; however, the potential cytotoxicity caused by negatively and particularly neutrally charged small CDs has been significantly overlooked. In this study, we conducted a comprehensive investigation into the cellular membrane disruption effect of weakly negatively charged 3-nm CDs using a combination of various biophysical techniques.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!