A total of 34 Staphylococcus aureus isolates from flock-wise pooled chicken neck skin samples collected at two abattoirs during slaughter were characterized with DNA microarray analysis and spa typing. The 20 isolates from abattoir A all belonged to clonal complex (CC) 12 and spa type t160. Of the 14 isolates from abattoir B, 7 belonged to CC5-t3478, 5 to CC12-t160, 1 to CC45-t040, and 1 to CC101-t056. Of the various resistance-associated genes tested, only blaZ/R/I (6 isolates of CC12 and CC101 from abattoir B), sdrM (n = 34), fosB (n = 33), and qacC (n = 22) were detected. None of the isolates harbored genes conferring methicillin resistance. In terms of genes encoding enterotoxins, seb (all isolates of CC12), egc (seg, sei, selm, seln, selo, selu; all isolates of CC5 and CC45), and sea (14 isolates of CC12 and 1 isolate of CC5) were found. In addition, all isolates harbored genes for intracellular adhesion proteins (icaA/C/D) and were positive for cap5 or cap8 (capsule type 5 or 8). Comparison of DNA microarray profiles identified four categories comprising (i) all isolates of CC12, (ii) all isolates of CC5, (iii) the CC45 isolate, and (iv) the CC101 isolate. The high similarity of the isolates from abattoir A could indicate contamination of chicken carcasses with S. aureus persisting on the slaughter equipment, but further investigations are required to elucidate potential contamination routes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4315/0362-028X.JFP-13-009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unveiling the genetic landscape of Streptococcus agalactiae bacteremia: emergence of hypervirulent CC1 strains and new CC283 strains in Tehran, Iran.
BMC Microbiol
September 2024
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Article Synopsis
- The study focuses on the increasing infections caused by Streptococcus agalactiae, highlighting the importance of understanding its epidemiology and virulence in patients with blood infections.
- Researchers analyzed 61 S. agalactiae isolates for antibiotic resistance and biofilm formation, finding that 32.7% of isolates showed clindamycin resistance and a significant portion demonstrated strong biofilm-forming abilities.
- Genetic analysis revealed the isolates belonged to six clonal complexes, with most of them being strong biofilm producers, while some complexes showed no biofilm production at all.
Characterization of Streptococcus agalactiae 1a isolated from farmed Nile tilapia (Oreochromis niloticus) in North America, Central America, and Southeast Asia.
Fish Shellfish Immunol
November 2024
Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway. Electronic address:
Article Synopsis
- Streptococcus agalactiae 1a causes severe disease in Nile tilapia, leading to increased antibiotic use in aquaculture, with no licensed vaccine currently available.
- This study analyzed isolates from North America, Central America, and Southeast Asia to assess their potential for vaccine development and found that the isolates share similar biochemical and antigenic profiles.
- The research indicates that vaccines using Streptococcus agalactiae 1a from these regions could effectively protect tilapia from the disease globally, suggesting a uniformity in the pathogens across different locations.
Genomic insights into the diversity, virulence, and antimicrobial resistance of group B clinical isolates from Saudi Arabia.
Front Cell Infect Microbiol
May 2024
Infectious Diseases Research Department, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.
Introduction: Detailed assessment of the population structure of group B (GBS) among adults is still lacking in Saudi Arabia. Here we characterized a representative collection of isolates from colonized and infected adults.
Methods: GBS isolates (n=89) were sequenced by Illumina and screened for virulence and antimicrobial resistance determinants.
Antibiotic Resistance Profiles and Molecular Characteristics of -Carrying Serovar Albany Isolated from Chickens During 2013-2020 in South Korea.
Foodborne Pathog Dis
November 2023
Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, Republic of Korea.
The production of β-lactamase by nontyphoidal has become a public health issue throughout the world. In this study, we aimed to investigate the antimicrobial resistance profiles and molecular characteristics of β-lactamase-producing serovar Albany isolates. A total of 434 Albany were obtained from feces and carcasses of healthy and diseased food-producing animals [cattle ( = 2), pigs ( = 3), chickens ( = 391), and ducks ( = 38)] during 2013-2020.
View Article and Find Full Text PDF[Population structure of food-borne in China].
Zhonghua Liu Xing Bing Xue Za Zhi
June 2023
Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China Chaoyang District Center for Disease Control and Prevention, Beijing 100020, China.
Article Synopsis
- - The study analyzed 763 food-borne strains from China between 2006 and 2020 using whole genome sequencing and various typing methods, revealing a total of 90 sequence types (STs), including 20 novel types.
- - The predominant clone complexes identified (CC7, CC1, CC5, CC398, CC188, and others) made up 82.44% of the strains, showing changes over the years, with a 7.60% detection rate of methicillin-resistant strains (MRSA).
- - A phylogenetic tree showed two clades, separating methicillin-sensitive strains from MRSA strains, with imported food strains demonstrating distinct genetic relationships from local strains, highlighting the need for
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!