AI Article Synopsis
- A new nucleic acid analogue called (S)-butyl nucleic acid (BuNA) was created, featuring a unique acyclic backbone with natural nucleobases.
- Incorporating (S)-BuNA into DNA strands showed that it forms stable duplexes without altering the typical B-type helical structure.
- Studies indicated that DNA polymerases can extend primers containing (S)-BuNA, suggesting the analogue's compatibility with biological processes.
Article Abstract
A novel nucleic acid analogue called acyclic (S)-butyl nucleic acid (BuNA) composed of an acyclic backbone containing a phosphodiester linkage and bearing natural nucleobases was synthesized. Next, (S)-BuNA nucleotides were incorporated in DNA strands and their effect on duplex stability and changes in structural conformation were investigated. Circular dichroism (CD), UV-melting and non-denatured gel electrophoresis (native PAGE) studies revealed that (S)-BuNA is capable of making duplexes with its complementary strands and integration of (S)-BuNA nucleotides into DNA duplex does not alter the B-type-helical structure of the duplex. Furthermore, (S)-BuNA oligonucleotides and (S)-BuNA substituted DNA strands were studied as primer extensions by DNA polymerases. This study revealed that the acyclic scaffold is tolerated by enzymes and is therefore to some extent biocompatible.
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| http://dx.doi.org/10.1039/c3ob41244j | DOI Listing |
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