Objective: To study the chemical constituents of petroleum ether portion from Seed of Picria felterrae.

Methods: The chemical constituents were extracted and isolated with solvent and chromatography, and the chemical structures were determined on the basis of physicochemical properties and spectral data (UV, IR, 1H-NMR, 13C-NMR, MS).

Results: Six chemical constituents were identified as follows: chrysophanol (I), 1-hydroxy-6-methyl-anthraquinone (II), beta-sitosterol (III), betulin (IV), N-benzoyl-phenylalanyl-L-phenylalaninol acetate (V) and picfeltarraenone I (VI).

Conclusion: Compounds I, II and IV are isolated from this plant for the first time.

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