By engineering a gold nanoparticle and thymine-Hg(2+)-thymine-based hairpin DNA probe, a gold nanocarrier-based strategy for in vitro detection and live-cell imaging of thiol-containing amino acids/peptides was proposed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c3cc44184a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cysteine import via the high-affinity GSH transporter Hgt1 rescues GSH auxotrophy in yeast.
J Biol Chem
December 2024
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina, USA. Electronic address:
Glutathione (GSH) is an abundant thiol-containing tripeptide that functions in redox homeostasis, protein folding, and iron (Fe) metabolism. In Saccharomyces cerevisiae, GSH depletion leads to increased sensitivity to oxidants and other toxic compounds, disruption of iron-sulfur (Fe-S) cluster biogenesis, and eventually cell death. GSH pools are supplied by intracellular biosynthesis and GSH import from the extracellular environment.
View Article and Find Full Text PDFSmartphone-integrated inkjet-printed paper sensor and UV/Vis spectrophotometric method for on-site detection of As and L-cysteine in food samples using novel AMTPP-functionalized silver nanoparticles.
Food Chem
February 2025
HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. Electronic address:
The increasing prevalence of arsenic (As) in water and its health impacts necessitate advanced detection methods. Similarly, monitoring L-Cysteine, a vital thiol-containing amino acid, is crucial for assessing physiological processes and disorders. This study presents a novel method for detecting As and L-Cysteine in food samples using 4-amino-3-(D-galactopentitol-1-yl)-5-mercapto-1,2,4-triazole (AMTPP) functionalized silver nanoparticles (AMTPP-AgNPs) by UV/Vis spectrophotometric method and smartphone-assisted inkjet-printed paper-based sensors.
View Article and Find Full Text PDFUltrasensitive Electrochemical Biosensor Based on Efficient PDA-APDMAO Antifouling Interface and Dual-Signal Ratio Strategy for Trace Detection of Alpha-Fetoprotein in Human Serum.
Anal Chem
September 2024
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Article Synopsis
- Developing biosensors that can effectively resist unwanted biomolecule adhesion in human serum is a major challenge, tackled using innovative antifouling materials like 3-aminopropyldimethylamine oxide (APDMAO), which creates a strong hydration barrier due to its unique charge properties.
- APDMAO enhances biocompatibility and eases the incorporation of aptamers and signal probes by copolymerizing with dopamine, facilitating a more efficient biosensor design without complex modifications.
- The resulting electrochemical biosensor demonstrates highly sensitive detection of alpha-fetoprotein (AFP), a liver cancer marker, with a detection range from 10 fg/mL to 10 ng/mL, highlighting its potential for clinical applications in cancer
The first ER-targeting flavone-based fluorescent probe for Cys: Applications in real-time tracking in an epilepsy model and food analysis.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Sichuan New Green Pharmaceutical Technology Development Co. Ltd., Chengdu 611930, China. Electronic address:
Article Synopsis
- Epilepsy is a common neurological disorder linked to endoplasmic reticulum stress (ERS) and oxidative stress (OS), with cysteine (Cys) playing a potential role in these processes.
- Researchers developed a new fluorescent probe called BFC that effectively targets the endoplasmic reticulum, allowing for quick and sensitive detection of Cys levels (3 minutes response time, low detection limit).
- The BFC probe demonstrated low toxicity and was successful in monitoring Cys levels in both zebrafish models of epilepsy and various food samples, even enabling smartphone-assisted detection for practical use.
Switching the Chemoselectivity in the Preparation of [F]FNA--CooP, a Free Thiol-Containing Peptide for Targeted Positron Emission Tomography Imaging of Fatty Acid Binding Protein 3.
Mol Pharm
August 2024
Turku PET Centre, University of Turku, Kiinamyllynkatu 4-8, Turku FI-20520, Finland.
Fatty acid binding protein 3 (FABP3) is expressed both in tumor cells and in the tumor vasculature, making it a potential target for medical imaging and therapy. In this study, we aimed to radiolabel a CooP peptide with a free amino and thiol group, and evaluate the radiolabeled product [F]FNA--CooP for imaging FABP3 expression in breast cancer brain metastases by positron emission tomography. [F]FNA--CooP was prepared by highly chemoselective -acylation and characterized using different chemical approaches.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!