Transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.

J Biotechnol

Laboratorio de Agrobiotecnología, Departamento de Fisiología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA Buenos Aires, Argentina.

Published: September 2013

AI Article Synopsis

  • Citrus canker, caused by the bacterium Xanthomonas axonopodis pv. citri, severely impacts citrus agriculture globally, particularly in Northeast and Northwest Argentina where it can affect 10% of plantations.
  • There is currently no known natural resistance to this disease in existing orange varieties or rootstocks.
  • Transgenic Pineapple sweet orange plants were developed to express dermaseptin, leading to a reduction in symptoms by up to 50% compared to non-transgenic plants in controlled testing.

Article Abstract

Citrus canker provoked by Xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. Xanthomonas infection is considered as an endemic disease in Northeast and Northwest Argentina, affecting as much as 10% of commercial citrus plantations. There is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. To introduce resistance to this disease, plants of Pineapple sweet orange were transformed with a genetic construct allowing constitutive accumulation of dermaseptin. In comparison with non-transformed plants, transgenic plants showed symptom reduction levels of up to 50% in in planta assays performed under controlled conditions.

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Source
http://dx.doi.org/10.1016/j.jbiotec.2013.07.019DOI Listing

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