Thermophilic anaerobic digestion of stillage from a cellulosic ethanol process that uses sugarcane bagasse as feedstock was investigated. A biochemical methane potential (BMP) of 200 ml CH4 at STP (g VS)(-1) was obtained. The whole stillage was separated into two fractions: a fraction retained on 0.5 mm screen called residue and a fraction passing through 0.5 mm screen called filtrate. About 70% of total methane yield of stillage was produced from the filtrate. The filtrate was anaerobically digested in a 15 L semi-continuously fed digester operated for 91 days at HRTs of 21 and 14 days and organic loading rate (OLR) of 1.85 and 2.39 g COD L(-1) d(-1). The methane yield from the stillage from the digester was about 90% of the yield from the BMP assays. The influent soluble COD (sCOD) was reduced from between 35.4 and 38.8 g COD (L(-1)) to between 7.5 and 8 g COD (L(-1)).
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