Crustins are cysteine-rich cationic antimicrobial peptides (AMPs) found in decapod crustaceans. Six novel crustin genes (Paj-CrusIc, Id, Ie, If, IIb and IIc) were identified in the morotoge shrimp, Pandalopsis japonica. Deduced amino acid sequences of isolated Paj-Crus genes ranged from 99 to 178 amino acid residues (10.6-17.8 kDa). Sequence analysis of nine isolated Paj-Crus genes and 100 different crustins from various decapod crustaceans revealed that a splice site and KXXXCP motif within the WAP domain may be the main criteria for classifying type I and II crustins, suggesting that the two types of crustin genes may have been generated by different processes. We also identified three intron-less crustin I genes (Paj-Crus Id, Ie and If) for the first time, which may have been generated by gene duplication. The tissue distribution profiles showed that Paj-CrusI genes were expressed predominantly in the gill and epidermis, whereas Paj-CrusII genes were expressed ubiquitously, suggesting that the two types of crustins may play different roles in various tissues or under different physiological conditions. Differing from previous results, hemocyte-specific crustin was not isolated from Pandalopsis japonica. This study showed that both types of crustin genes (types I and II) exist in decapod crustaceans and their primary structure and expression profiles differ from each other, suggesting that they may play different biological roles. This will help to extend our knowledge of the crustacean innate immune response, which will provide important basic information of shrimp immunity against various pathogens.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2013.07.031 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ocean warming and acidification alter calcification and innate immune system gene expression in juvenile American lobsters, Homarus americanus.
Comp Biochem Physiol Part D Genomics Proteomics
December 2024
School for the Environment, University of Massachusetts Boston, Boston, MA 02125, USA; Presidents Office, Ursinus College, Collegeville, PA 19426, USA.
Article Synopsis
- The Gulf of Maine is facing severe ocean warming and acidification, impacting the American lobster, with limited research on how these stressors interact specifically on gene expression.
- Two aquarium experiments were conducted that simulated future ocean conditions to assess the effects of these stressors on juvenile lobsters' gene expression, revealing complex responses in genes related to calcification and immune function.
- Findings indicate that elevated temperatures significantly affect gene expression, potentially leading to impaired normal development and increased vulnerability to disease due to these combined stressors.
Effects of dietary probiotic ( I9, G15, or X13) on growth, digestive enzyme activities, immunity, and intestinal microbiota of Pacific white shrimp ().
Front Microbiol
November 2024
Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China.
Pacific white shrimp () is one of the most productive and economically important species globally. However, the development and continuous expansion of the farming scale led to an increase in the risk of disease occurrence in shrimp farming. The application of probiotics as an effective method for controlling diseases in aquaculture has been widely considered.
View Article and Find Full Text PDFEffects of Fermented Pomegranate Peel Polyphenols on the Growth Performance, Immune Response, Hepatopancreatic Health, and Disease Resistance in White Shrimp ().
Aquac Nutr
November 2024
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
This study evaluated the growth performance, immune response, hepatopancreatic health, and disease resistance in fed diets supplemented with fermented pomegranate peel polyphenols (FPPP) for 45 days. Five diets were formulated to contain various levels of FPPP: FP0 (no FPPP), FPPP inclusion at 0.015% (FP1), 0.
View Article and Find Full Text PDFBinding of Infectious Hypodermal and Haematopoietic Necrosis Virus-Like Particles to Mannose Receptor Stimulates Antimicrobial Responses in Immune-Related Tissues of Peneaus vannamei.
J Fish Dis
November 2024
School of Medicine, Walailak University, Nakhonsrithammarat, Thailand.
Mannose receptor (MR) is a transmembrane protein and a type of pattern-recognition receptor (PRR) that plays a critical role in the immunity of mammals and fish. In this study, we examined the role of MR in binding with infectious hypodermal and haematopoietic necrosis virus-like particle (IHHN-VLP) and the downstream immune pathway that it triggers in the shrimp Peneaus vannamei. Upon IHHN-VLP challenge, transcripts of MR in P.
View Article and Find Full Text PDFPolystyrene nanoplastics induce apoptosis, histopathological damage, and glutathione metabolism disorder in the intestine of juvenile East Asian river prawns (Macrobrachium nipponense).
Sci Total Environ
December 2024
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China. Electronic address:
Nanoplastics (NPs) are widely distributed in the aquatic environment and have become a global concern as a new type of pollutant. Many researchers have studied the physiological effects of NPs on aquatic organisms, but relatively little is known about their effects on intestinal immune function in crustaceans. Therefore, we used NPs concentrations of 0, 5, 10, 20, 40 mg/L for 28 days of stress, evaluated the effects of NPs exposure on intestinal cell apoptosis, histopathological damage, and glutathione (GSH) metabolism of juvenile East Asian river prawns (Macrobrachium nipponense).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!