Introduction: It is known that sexual assault victim's contacting to water may decrease the detectability of sperm cells on the body of a victim. The features of water (i.e. chemical and biological) also have an importance in this respect. In this study, it is aimed at studying different kinds of water in terms of the detection of sperm cells in vitro. The difference of the study from other studies in the literature is the usage of different types of water and the search for detectability of sperm in these water samples regarding both time and dilution factor.
Materials And Methods: After sexual abstinence, 30 volunteer young adults' ejaculate samples were taken with their informed consent. These samples were examined in liquid media in terms of change in time. Ejaculate samples which were standardized as to include 50 million sperms cells in each milliliter were diluted by 20% in 4 tubes including distilled water, tap water, sea water and river water. Sperm count in diluted samples was conducted daily.
Results: It is determined that sperm cells can be detected after a longer time in tap and distilled water than in sea and fresh water. Sperm cells were detected up to 1128 h in tap water, 888 h in sea water, 1008 h in distilled water and 792 h in river water. Changes in sperm count in terms of dilution rate and liquid type were recorded.
Discussion And Conclusion: It is known that in vitro results are not the same as in vivo results. Time-dependent changes about detectability of sperm cells in different types of water are presented in tables and figures.
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http://dx.doi.org/10.1016/j.forsciint.2013.04.037 | DOI Listing |
Narra J
December 2024
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Medicinal herbs, such as the ant nest plant (), are promising for the management of diabetes mellitus-associated infertility. The aim of this study was to evaluate the biological activity of the ant nest plant and its capacity to mitigate the adverse effects of alloxan-induced diabetes on testicular morphology, epididymal function, and sperm quality in male rats. The tuber of the ant nest plant was extracted using methanol and then subjected to phytochemical screenings.
View Article and Find Full Text PDFBasic Clin Pharmacol Toxicol
February 2025
Department of Pharmacology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Paclitaxel (PAC), derived from Taxus brevifolia, is used to treat solid tumours but causes reproductive toxicity due to oxidative stress, affecting sperm quality and testicular tissue. Nerolidol (NRL), an antioxidant sesquiterpene alcohol, has not been studied for its potential to reduce PAC-induced reproductive damage. This study investigates NRL's ability to mitigate PAC-induced reproductive toxicity in rats.
View Article and Find Full Text PDFEpigenetics Chromatin
January 2025
Department of Maternal‑Fetal Biology, National Center for Child Health and Development, Tokyo, 157‑8535, Japan.
Background: DNA methylation plays a crucial role in mammalian development. While methylome changes acquired in the parental genomes are believed to be erased by epigenetic reprogramming, accumulating evidence suggests that methylome changes in sperm caused by environmental factors are involved in the disease phenotypes of the offspring. These findings imply that acquired sperm methylome changes are transferred to the embryo after epigenetic reprogramming.
View Article and Find Full Text PDFBiotech Histochem
January 2025
Faculty of Medicine, Department of Medical Pathology, Sivas Cumhuriyet University, Sivas, Turkey.
The main symptoms of depression, a chronic mental illness, include sadness, low self-esteem, and a diminished sense of enjoyment in life. Many factors have been suggested to be associated with depression, one of which is low testosterone in men. The serotonin reuptake inhibitor fluoxetine (FLU), used to treat depression, has been reported to potentially have detrimental effects on spermatogenesis in rats after long-term use.
View Article and Find Full Text PDFToxicol Rep
June 2025
Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, El Buhouth St., Dokki, Cairo 12622, Egypt.
Doxorubicin (DOX) is a powerful antineoplastic FDA-approved anthracycline-derived antibiotic and is considered as the most suitable intervention for solid tumors and hematological cancers therapy. However, its therapeutic application is highly limited due to acute and chronic renal, hematological and testicular toxicity. Oxidative stress, lipid peroxidation and apoptosis in germ cells as well as low sperm count, motility and disturbing steroidogenesis are the principal machineries of DOX-induced testicular toxicity.
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