AI Article Synopsis
- A new multiphoton excitation system utilizes temporal focusing and patterned laser control for rapid creation of 3D gray-level biomicrostructures through two-photon cross-linking with rose bengal as a photoactivator.
- Different concentrations of bovine serum albumin (BSA) structures can be produced simultaneously by adjusting pulse numbers and laser power, achieving results in just seconds.
- The intensity of two-photon excited fluorescence from rose bengal serves as a real-time monitoring tool for assessing the concentration of cross-linked BSA microstructures, making this method beneficial for biomedical research on extracellular matrices.
Article Abstract
ABSTRACT. A developed temporal focusing-based multiphoton excitation system with additional patterned excitation and local laser control can provide high-throughput fabrication of three-dimensional gray-level biomicrostructures via two-photon cross-linking with rose bengal (RB) as the photoactivator. Multiple bovine serum albumin (BSA) structures of different concentrations were simultaneously achieved by selecting different pulse numbers in the designated regions with an appropriate femtosecond laser power within a few seconds. Intensity of the RB two-photon excited fluorescence (TPEF) is correlated to the concentration of the fabricated cross-linked BSA microstructure. Hence, the fabricated BSA microstructure can be monitored online by utilizing the RB TPEF as contrast agent. It suggests that this approach with its unique capability of high-speed, gray-level, and online-inspection fabrication meets the requirements of the biomedical researches involved in extracellular matrix.
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| http://dx.doi.org/10.1117/1.JBO.18.7.075004 | DOI Listing |
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